Specifications
| Size | 50μL/100μL |
| Target | ID4 |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC, IF, ELISA |
| MW(Calculated) | 17kD |
| MW(Observed) | 20kD |
| Host Species | Rabbit |
| Isotype | IgG,Kappa |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | IHC 1:200-1:1000; WB 1:2000-1:10000; IF 1:200-1:1000; ELISA 1:5000-1:20000; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Purification | Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | RM1832R |
| Immunogen | The specific immunogen used to produce this antibody is proprietary information. |
| Specificity | Endogenous |
| Gene Name | ID4 |
| Protein Name | DNA-binding protein inhibitor ID-4 |
| Other Name | ID4; / BHLHB27; / DNA-binding protein inhibitor ID-4; / Class B basic helix-loop-helix protein 27; / bHLHb27; / Inhibitor of DNA binding 4 |
| Species | Human |
| Gene ID | 3400 |
| Uniprot | P47928, |
| Species.1 | Mouse |
| Gene ID.1 | 15904 |
| Uniprot.1 | P41139 |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | Nucleus. |
| Tissue Expression | Abdominal adipose tissue,Uterus, |
| Signaling_pathway | Cellular Processes >> Cellular community - eukaryotes >> Signaling pathways regulating pluripotency of stem cells;Environmental Information Processing >> Signal transduction >> TGF-beta signaling pathway |
| Research Areas | >>TGF-beta signaling pathway; / >>Signaling pathways regulating pluripotency of stem cells |
| Function | Function:ID (inhibitor of DNA binding) HLH proteins lack a basic DNA-binding domain but are able to form heterodimers with other HLH proteins, thereby inhibiting DNA binding.,similarity:Contains 1 basic helix-loop-helix (bHLH) domain.,subunit:Heterodimer with other HLH proteins., |
