Price range: $268.00 through $328.00
Background：
Neurofilaments are type IV intermediate filament heteropolymers composed of light , medium , and heavy chains. Neurofilaments comprise the axoskeleton and they functionally maintain the neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene is a member of the intermediate filament family and is involved in the morphogenesis of neurons. [provided by RefSeq , Jun 2009] ,
| Size | 50μL/100μL |
| Target | Internexin-α |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC, IF, IP, ELISA |
| MW(Calculated) | 52kD |
| MW(Observed) | 66kD |
| Host Species | Rabbit |
| Isotype | IgG,Kappa |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | IHC 1:200-1:1000; WB 1:2000-1:10000; IF 1:200-1:1000; ELISA 1:5000-1:20000; IP 1:50-1:200; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Purification | Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | RM2006R |
| Immunogen | The specific immunogen used to produce this antibody is proprietary information. |
| Specificity | Endogenous |
| Gene Name | INA |
| Protein Name | Alpha-internexin |
| Other Name | INA; / NEF5; / Alpha-internexin; / Alpha-Inx; / 66 kDa neurofilament protein; / NF-66; / Neurofilament-66; / Neurofilament 5 |
| Species | Human |
| Gene ID | 9118 |
| Uniprot | Q16352, |
| Species.1 | Mouse |
| Gene ID.1 | 226180 |
| Uniprot.1 | P46660, |
| Species.2 | Rat |
| Gene ID.2 | 24503 |
| Uniprot.2 | P23565 |
| Cellular Localization | extracellular space,nucleoplasm,neurofilament,nuclear membrane,cytoplasmic ribonucleoprotein granule,myelin sheath,intermediate filament cytoskeleton, |
| Tissue Expression | Found predominantly in adult CNS. |
| Signaling_pathway | |
| Research Areas | |
| Function | developmental stage:Expressed in brain as early as the 16th week of gestation, and increased rapidly and reached a steady state level by the 18th week of gestation.,Function:Class-IV neuronal intermediate filament that is able to self-assemble. It is involved in the morphogenesis of neurons. It may form an independent structural network without the involvement of other neurofilaments or it may cooperate with NF-L to form the filamentous backbone to which NF-M and NF-H attach to form the cross-bridges.,PTM:O-glycosylated.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Belongs to the intermediate filament family.,tissue specificity:Found predominantly in adult CNS., |
