U-Blot® JAM-A Rabbit mAb
Ucallm_WR5333-50
Price range: $268.00 through $328.00
Product details
Background:Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. The protein encoded by this immunoglobulin superfamily gene member is an important regulator of tight junction assembly in epithelia. In addition, the encoded protein can act as (1) a receptor for reovirus, (2) a ligand for the integrin LFA1, involved in leukocyte transmigration, and (3) a platelet receptor. Multiple 5' alternatively spliced variants, encoding the same protein, have been identified but their biological validity has not been established. [provided by RefSeq, Jul 2008],
Specifications
| Size | 50μL/100μL |
| Target | JAM-A |
| Reactivity | Human |
| Applications | WB, IHC, IF, ELISA |
| MW(Calculated) | 33kD |
| MW(Observed) | 38kD |
| Host Species | Rabbit |
| Isotype | IgG,Kappa |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | IHC 1:200-1:1000; WB 1:2000-1:10000; IF 1:200-1:1000; ELISA 1:5000-1:20000; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Purification | Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | RM1567R |
| Immunogen | The specific immunogen used to produce this antibody is proprietary information. |
| Specificity | Endogenous |
| Gene Name | F11R |
| Protein Name | Junctional adhesion molecule A |
| Other Name | F11R; / JAM1; / JCAM; / Junctional adhesion molecule A; / JAM-A; / Junctional adhesion molecule 1; / JAM-1; / Platelet F11 receptor; / Platelet adhesion molecule 1; / PAM-1; / CD321 |
| Species | Human |
| Gene ID | 50848 |
| Uniprot | Q9Y624, |
| Species.1 | Mouse |
| Gene ID.1 | 16456 |
| Uniprot.1 | O88792, |
| Species.2 | Rat |
| Gene ID.2 | 116479 |
| Uniprot.2 | Q9JHY1 |
| Cellular Localization | Cell junction, tight junction . Cell membrane; Single-pass type I membrane protein . Localized at tight junctions of both epithelial and endothelial cells. . |
| Tissue Expression | Expressed in endothelium, epithelium and leukocytes (at protein level). |
| Signaling_pathway | Cellular Processes >> Cellular community - eukaryotes >> Tight junction;Organismal Systems >> Immune system >> Leukocyte transendothelial migration;Environmental Information Processing >> Signaling molecules and interaction >> Cell adhesion molecules |
| Research Areas | >>Cell adhesion molecules; / >>Tight junction; / >>Leukocyte transendothelial migration; / >>Epithelial cell signaling in Helicobacter pylori infection |
| Function | Function:Seems to plays a role in epithelial tight junction formation. Appears early in primordial forms of cell junctions and recruits PARD3. The association of the PARD6-PARD3 complex may prevent the interaction of PARD3 with JAM1, thereby preventing tight junction assembly (By similarity). Plays a role in regulating monocyte transmigration involved in integrity of epithelial barrier. Involved in platelet activation. In case of orthoreovirus infection, serves as receptor for the virus.,PTM:N-glycosylated.,similarity:Belongs to the immunoglobulin superfamily.,similarity:Contains 2 Ig-like V-type (immunoglobulin-like) domains.,subcellular location:Localized at tight junctions of both epithelial and endothelial cells.,subunit:Interacts with the ninth PDZ domain of MPDZ. Interacts with the first PDZ domain of PARD3. The association between PARD3 and PARD6B probably disrupts this interaction. Interacts with the orthoreovirus sigma-1 capsid protein., |
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