U-Blot® LATS2 Rabbit mAb
Ucallm_WR5292-50
Price range: $268.00 through $328.00
Product details
Background:This gene encodes a serine/threonine protein kinase belonging to the LATS tumor suppressor family. The protein localizes to centrosomes during interphase, and early and late metaphase. It interacts with the centrosomal proteins aurora-A and ajuba and is required for accumulation of gamma-tubulin and spindle formation at the onset of mitosis. It also interacts with a negative regulator of p53 and may function in a positive feedback loop with p53 that responds to cytoskeleton damage. Additionally, it can function as a co-repressor of androgen-responsive gene expression. [provided by RefSeq, Jul 2008],
Specifications
| Size | 50μL/100μL |
| Target | LATS2 |
| Reactivity | Human |
| Applications | WB, IF, ELISA |
| MW(Calculated) | 120kD |
| MW(Observed) | 130kD |
| Host Species | Rabbit |
| Isotype | IgG,Kappa |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:1000-1:5000; IF 1:200-1:1000; ELISA 1:5000-1:20000; |
| Formulation | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Purification | Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | RM1587R |
| Immunogen | The specific immunogen used to produce this antibody is proprietary information. |
| Specificity | Endogenous |
| Gene Name | LATS2 |
| Protein Name | Serine/threonine-protein kinase LATS2 |
| Other Name | LATS2; / KPM; / Serine/threonine-protein kinase LATS2; / Kinase phosphorylated during mitosis protein; / Large tumor suppressor homolog 2; / Serine/threonine-protein kinase kpm; / Warts-like kinase |
| Species | Human |
| Gene ID | 26524 |
| Uniprot | Q9NRM7, |
| Species.1 | Mouse |
| Gene ID.1 | 50523 |
| Uniprot.1 | Q7TSJ6 |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm. Cytoplasm, cytoskeleton, spindle pole. Nucleus. Colocalizes with AURKA at the centrosomes during interphase, early prophase and cytokinesis. Migrates to the spindle poles during mitosis, and to the midbody during cytokinesis. Translocates to the nucleus upon mitotic stress by nocodazole treatment. |
| Tissue Expression | Expressed at high levels in heart and skeletal muscle and at lower levels in all other tissues examined. |
| Signaling_pathway | Environmental Information Processing >> Signal transduction >> Hippo signaling pathway;Environmental Information Processing >> Signal transduction >> Hippo signaling pathway - multiple species |
| Research Areas | >>Hippo signaling pathway; / >>Hippo signaling pathway - multiple species |
| Function | Catalytic activity:ATP + a protein = ADP + a phosphoprotein.,cofactor:Magnesium.,Function:Tumor suppressor which plays a critical role in centrosome duplication, maintenance of mitotic fidelity and genomic stability. Negatively regulates G1/S transition by down-regulating cyclin E/CDK2 kinase activity. Negative regulator of the androgen receptor.,PTM:Autophosphorylated and phosphorylated during M-phase and the G1/S-phase of the cell cycle. Phosphorylated and activated by STK3.,similarity:Belongs to the protein kinase superfamily.,similarity:Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.,similarity:Contains 1 AGC-kinase C-terminal domain.,similarity:Contains 1 protein kinase domain.,similarity:Contains 1 UBA domain.,subcellular location:Co-localizes with STK6 at the centrosomes during interphase, early prophase and cytokinesis. Migrates to the spindle poles during mitosis, and to the midbody during cytokinesis.,subunit:Interacts with and is phosphorylated by STK6. Binds to AR. Interacts with JUB during mitosis and this complex regulates organization of the spindle apparatus through recruitment of gamma-tubulin to the centrosome.,tissue specificity:Expressed at high levels in heart and skeletal muscle and at lower levels in all other tissues examined., |
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