Specifications
| Size | 50μL/100μL |
| Target | MYH10 |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC, IF, IP, ELISA |
| MW(Calculated) | 229kD |
| MW(Observed) | 229kD |
| Host Species | Rabbit |
| Isotype | IgG,Kappa |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | IHC 1:500-1:2000; WB 1:2000-1:10000; IF 1:200-1:1000; ELISA 1:5000-1:20000; IP 1:50-1:200; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Purification | Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | RM1265R |
| Immunogen | The specific immunogen used to produce this antibody is proprietary information. |
| Specificity | Endogenous |
| Gene Name | MYH10 |
| Protein Name | Myosin-10 (Cellular myosin heavy chain, type B) (Myosin heavy chain 10) (Myosin heavy chain, non-muscle IIb) (Non-muscle myosin heavy chain B) (NMMHC-B) (Non-muscle myosin heavy chain IIb) (NMMHC II-b) (NMMHC-IIB) |
| Other Name | |
| Species | Human |
| Gene ID | 4628 |
| Uniprot | P35580, |
| Species.1 | Mouse |
| Gene ID.1 | 77579 |
| Uniprot.1 | Q61879, |
| Species.2 | Rat |
| Gene ID.2 | 79433 |
| Uniprot.2 | Q9JLT0 |
| Cellular Localization | Cell projection, lamellipodium . Colocalizes with MCC at the leading edge of migrating cells. |
| Tissue Expression | Isoform 1 is expressed in cerebellum and spinal chord. Isoform 2 is expressed in cerebrum and retina. Isoform 3 is expressed in the cerebrum and to a much lower extent in cerebellum. |
| Signaling_pathway | Cellular Processes >> Cellular community - eukaryotes >> Tight junction;Cellular Processes >> Cell motility >> Regulation of actin cytoskeleton;Organismal Systems >> Circulatory system >> Vascular smooth muscle contraction |
| Research Areas | |
| Function | Cellular myosin that appears to play a role in cytokinesis, cell shape, and specialized functions such as secretion and capping. Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2. During cell spreading, plays an important role in cytoskeleton reorganization, focal contacts formation (in the central part but not the margins of spreading cells), and lamellipodial extension; this function is mechanically antagonized by MYH9. |
