SKU: WR5937 Categories: ,

U-Blot® p57kip2 Rabbit mAb

Price range: $268.00 through $328.00

SKU: WR5937-50
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Product details

Background:
P57kip2 is a cell cycle inhibitor and tumor suppressor. The gene is mainly located on the mother’s chromosome 11. By blocking the G1 / S phase transition in the cell cycle , we can realize the negative regulation of the cell cycle , and then prevent cell proliferation and tumor formation. In complete hydatidiform mole , there is a lack of maternal gene and p57 negative expression , while in partial hydatidiform mole , there are genes of both parents and p57 positive expression , which can be used for the study of early hydatidiform mole pregnancy.

Specifications

Size50μL/100μL
Targetp57
ReactivityHuman, Mouse, Rat
ApplicationsWB, IHC, IF, IP, ELISA
MW(Calculated)32kD
MW(Observed)52kD
Host SpeciesRabbit
IsotypeIgG,Kappa
Conjugate/ModificationUnmodified
Recommended Dilution RatioIHC 1:200-1:1000; WB 1:5000-1:20000; IF 1:200-1:1000; ELISA 1:5000-1:20000; IP 1:50-1:200; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004)
FormulationPBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
PurificationRecombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography.
Storage-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration
ClonalityMonoclonal
Clone NumberRM1900R
ImmunogenThe specific immunogen used to produce this antibody is proprietary information.
SpecificityEndogenous
Gene NameCDKN1C
Protein NameCyclin-dependent kinase inhibitor 1C
Other NameBeckwith Wiedemann syndrome; / BWCR; / BWS; / CDKI; / CDKN 1C; / CDKN1C; / CDN1C_HUMAN; / Cyclin dependent kinase inhibitor 1C; / Cyclin dependent kinase inhibitor p57; / Cyclin-dependent kinase inhibitor 1C; / Cyclin-dependent kinase inhibitor p57; / KIP 2; / KIP2; / p57; / p57 Kip 2; / p57KIP2; / WBS
SpeciesHuman
Gene ID1028
UniprotP49918,
Species.1Mouse
Gene ID.112577
Uniprot.1P49919
Species.2
Gene ID.2
Uniprot.2
Cellular LocalizationNuclear
Tissue ExpressionExpressed in the heart, brain, lung, skeletal muscle, kidney, pancreas and testis. Expressed in the eye. High levels are seen in the placenta while low levels are seen in the liver.
Signaling_pathwayCellular Processes >> Cell growth and death >> Cell cycle
Research Areas>>Cell cycle
FunctionDisease:Defects in CDKN1C are a cause of Beckwith-Wiedemann syndrome (BWS) [MIM:130650]. BWS is a genetically heterogeneous disorder characterized by anterior abdominal wall defects including exomphalos (omphalocele), pre- and postnatal overgrowth, and macroglossia. Additional less frequent complications include specific developmental defects and a predisposition to embryonal tumors.,Disease:Defects in CDKN1C are involved in tumor formation.,Function:Potent tight-binding inhibitor of several G1 cyclin/CDK complexes (cyclin E-CDK2, cyclin D2-CDK4, and cyclin A-CDK2) and, to lesser extent, of the mitotic cyclin B-CDC2. Negative regulator of cell proliferation. May play a role in maintenance of the non-proliferative state throughout life.,similarity:Belongs to the CDI family.,tissue specificity:Expressed in the heart, brain, lung, skeletal muscle, kidney, pancreas and testis. High levels are seen in the placenta while low levels are seen in the liver.,
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