$999.00
The affinity purified rabbit anti-phospho-tyrosine antibody was conjugated to d-biotin via N-acetylation. The conjugates are useful for the detection, identification, and quantization of tyrosine-phosphorylated proteins and peptides. These conjugates have been utilized for non-radioactive tyrosine kinase assays.
| Target | Phospho-Tyrosine |
| Reactivity | Species Independent |
| Application | ELISA、WB、IP、IHC |
| MW(Calculated) | Multiple |
| MW(Observed) | Multiple |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate | Biotin |
| Modification | Tyrosine Phosphorylation |
| Modified site | Tyr（Y） |
| Recommended dilution ratio | ELISA: 0.25 - 1.0 µg/mL；WB: 0.25 - 1.0 µg/mL；IP: 5 µg/mg protein；IHC: 10 µg/mL |
| Composition | 250 µg/mL in PBS, 50% glycerol |
| Purification process | The antibody was affinity purified with phospho-tyrosine immobilized on agarose. |
| Purity | |
| Storage | Product is stable for several weeks at 4°C. For extended storage, aliquot content and store product at –20ºC. Avoid cycles of freezing and thawing |
| Stability | Expiration date is one year from date of shipping if properly stored. |
| Clonal | Polyclonal |
| Clone Number | |
| Immunogen | Iodoacetylphosphotyamine-KLH conjugates |
| Sequence | |
| Specificity | |
| Gene name | |
| Protein Name | |
| Alternative name | P-Tyr |
| Organism-1 | |
| GeneID-1 | |
| SwissProt-1 | |
| Organism-2 | |
| GeneID-2 | |
| SwissProt-2 | |
| Organism-3 | |
| GeneID-3 | |
| SwissProt-3 | |
| Organism-4 | |
| GeneID-4 | |
| SwissProt-4 | |
| Background | Phospho-Tyrosine (pY) is a widely occurring post-translational modification on tyrosine residues in eukaryotic cells. This modification is catalyzed by protein tyrosine kinases (PTKs), which covalently attach a phosphate group to the phenolic hydroxyl group of tyrosine, and can be reversibly removed by protein tyrosine phosphatases (PTPs). Tyrosine phosphorylation serves as a core mechanism in cellular signal transduction, regulating cell proliferation, differentiation, apoptosis, migration, and metabolism. Dysregulation of tyrosine phosphorylation is closely associated with tumorigenesis, immune disorders, neurodegenerative diseases, and other pathological conditions, making it a critical target in cell signaling and therapeutic drug development. |
| Cellular Localization |
