U-Blot® Pan-Tri-Methyllysine Rabbit pAb，FITC
Ucallm_WT9184F-100
$999.00
Product details
This rabbit polyclonal antibody is raised with a chemically methylated protein antigen. The specific anti-tri-methyllysine antibody was affinity purified with trimethylated lysine antibodies and affinity adsorbed with N-(epsilon) mono- and di-methylated lysine. The purified anti-trimethyl-lysine antibody was labeled with FITC. The antibody could be utilized for detection and localization of proteins with N-trimethylated lysine residues.
Specifications
| Target | Tri-Methyllysine |
| Reactivity | Human and mouse |
| Application | For direct immunofluorescence experiments |
| MW(Calculated) | |
| MW(Observed) | |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate | Flourescein 5(6) isothionate; FITC/Ab molar ratio - >20:1 |
| Modification | Lysine Trimethylation |
| Modified site | Lys（K） |
| Recommended dilution ratio | |
| Composition | 250 ug/mL in PBS with 50% glycerol |
| Purification process | The antibody was purified on N (epsilon) tri-methyl lysine agarose and absorbed by mono- and di-methyl lysine. |
| Purity | |
| Storage | Product is stable for several weeks at 4°C. For extended storage, store product at –20ºC. |
| Stability | Expiration date is six months from date of shipping if properly stored. |
| Clonal | Polyclonal |
| Clone Number | |
| Immunogen | Methylated KLH conjugates |
| Sequence | |
| Specificity | |
| Gene name | |
| Protein Name | |
| Alternative name | Kme3 |
| Organism-1 | |
| GeneID-1 | |
| SwissProt-1 | |
| Organism-2 | |
| GeneID-2 | |
| SwissProt-2 | |
| Organism-3 | |
| GeneID-3 | |
| SwissProt-3 | |
| Organism-4 | |
| GeneID-4 | |
| SwissProt-4 | |
| Background | Histones undergo a wide range of enzyme-catalyzed post-translational modifications, including acetylation, methylation, phosphorylation and ubiquitination. Lysine trimethylation is a vital epigenetic modification that predominantly occurs on histone lysine residues. It is dynamically regulated by histone methyltransferases and demethylases, and extensively distributed in eukaryotic chromatin.Lysine trimethylation participates in governing chromatin structure remodeling, transcriptional activation or repression, cell cycle regulation, and DNA damage repair. Abnormal trimethylation levels are closely associated with tumorigenesis, developmental disorders, inflammation and neurodegenerative diseases, acting as a pivotal molecular marker for epigenetic research and disease mechanism investigation. |
| Cellular Localization |
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