Specifications
| Size | 50μL/100μL |
| Target | Pin1 |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC, IF, IP, ELISA |
| MW(Calculated) | 18kD |
| MW(Observed) | 18kD |
| Host Species | Rabbit |
| Isotype | IgG,Kappa |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | IHC 1:200-1:1000; WB 1:2000-1:10000; IF 1:200-1:1000; ELISA 1:5000-1:20000; IP 1:50-1:200; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Purification | Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | RM1743R |
| Immunogen | The specific immunogen used to produce this antibody is proprietary information. |
| Specificity | Endogenous |
| Gene Name | PIN1 |
| Protein Name | Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1 |
| Other Name | PIN1; / Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1; / Peptidyl-prolyl cis-trans isomerase Pin1; / PPIase Pin1; / Rotamase Pin1 |
| Species | Human |
| Gene ID | 5300 |
| Uniprot | Q13526, |
| Species.1 | Mouse |
| Gene ID.1 | 23988 |
| Uniprot.1 | Q9QUR7 |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | Nucleus . Nucleus speckle . Cytoplasm . Colocalizes with NEK6 in the nucleus (PubMed:16476580). Mainly localized in the nucleus but phosphorylation at Ser-71 by DAPK1 results in inhibition of its nuclear localization (PubMed:21497122). . |
| Tissue Expression | Expressed in immune cells in the lung (at protein level) (PubMed:29686383). The phosphorylated form at Ser-71 is expressed in normal breast tissue cells but not in breast cancer cells. |
| Signaling_pathway | Organismal Systems >> Immune system >> RIG-I-like receptor signaling pathway |
| Research Areas | >>Viral life cycle - HIV-1; / >>RIG-I-like receptor signaling pathway |
| Function | Catalytic activity:Peptidylproline (omega=180) = peptidylproline (omega=0).,Domain:The WW domain is required for the interaction with STIL and MPHOSPH1.,Function:Essential PPIase that regulates mitosis presumably by interacting with NIMA and attenuating its mitosis-promoting activity. Displays a preference for an acidic residue N-terminal to the isomerized proline bond. Catalyzing pSer/Thr-Pro cis/trans isomerizations.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Contains 1 PpiC domain.,similarity:Contains 1 WW domain.,subunit:Interacts with STIL (By similarity). Interacts with MPHOSPH1., |
