Name: U-Blot® PKD2 Rabbit mAb
SKU: WR5434
Price: 268 USD
Availability: InStock

U-Blot® PKD2 Rabbit mAb

Ucallm_WR5434-50

Price range: $268.00 through $328.00

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50μl

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SKU: WR5434-50

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Product details

Background:The protein encoded by this gene belongs to the protein kinase D (PKD) family of serine/threonine protein kinases. This kinase can be activated by phorbol esters as well as by gastrin via the cholecystokinin B receptor (CCKBR) in gastric cancer cells. It can bind to diacylglycerol (DAG) in the trans-Golgi network (TGN) and may regulate basolateral membrane protein exit from TGN. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008],

Specifications

Size	50μL/100μL
Target	PKD2
Reactivity	Human, Mouse
Applications	WB, IHC, IF, ELISA
MW(Calculated)	97kD
MW(Observed)	115kD
Host Species	Rabbit
Isotype	IgG,Kappa
Conjugate/Modification	Unmodified
Recommended Dilution Ratio	IHC 1:200-1:500; WB 1:2000-1:10000; IF 1:200-1:1000; ELISA 1:5000-1:20000; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004)
Formulation	PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
Purification	Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography.
Storage	-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration
Clonality	Monoclonal
Clone Number	RM1685R
Immunogen	The specific immunogen used to produce this antibody is proprietary information.
Specificity	Endogenous
Gene Name	PRKD2
Protein Name	Serine/threonine-protein kinase D2
Other Name	PRKD2; / PKD2; / HSPC187; / Serine/threonine-protein kinase D2; / nPKC-D2
Species	Human
Gene ID	25865
Uniprot	Q9BZL6,
Species.1	Mouse
Gene ID.1	101540
Uniprot.1	Q8BZ03,
Species.2	Rat
Gene ID.2	292658
Uniprot.2	Q5XIS9
Cellular Localization	Cytoplasm . Cell membrane . Nucleus . Golgi apparatus, trans-Golgi network . Translocation to the cell membrane is required for kinase activation. Accumulates in the nucleus upon CK1-mediated phosphorylation after activation of G-protein-coupled receptors. Nuclear accumulation is regulated by blocking nuclear export of active PRKD2 rather than by increasing import. .
Tissue Expression	Widely expressed.
Signaling_pathway	Organismal Systems >> Endocrine system >> Aldosterone synthesis and secretion;Environmental Information Processing >> Signal transduction >> Rap1 signaling pathway
Research Areas	>>Rap1 signaling pathway; / >>Aldosterone synthesis and secretion; / >>Chemical carcinogenesis - reactive oxygen species
Function	Catalytic activity:ATP + a protein = ADP + a phosphoprotein.,enzyme regulation:Activated by diacylglycerol and phorbol esters.,Function:Calcium-independent, phospholipid-dependent, serine- and threonine-specific protein kinase.,PTM:Autophosphorylated. Phorbol esters stimulates autophosphorylation. Phosphorylation of Ser-876 correlates with the activation status of the kinase.,similarity:Belongs to the protein kinase superfamily.,similarity:Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. PKD subfamily.,similarity:Contains 1 PH domain.,similarity:Contains 1 protein kinase domain.,similarity:Contains 2 phorbol-ester/DAG-type zinc fingers.,tissue specificity:Widely expressed.,