U-Blot® PKD2 Rabbit mAb
Ucallm_WR5434-50
Price range: $268.00 through $328.00
Product details
Background:The protein encoded by this gene belongs to the protein kinase D (PKD) family of serine/threonine protein kinases. This kinase can be activated by phorbol esters as well as by gastrin via the cholecystokinin B receptor (CCKBR) in gastric cancer cells. It can bind to diacylglycerol (DAG) in the trans-Golgi network (TGN) and may regulate basolateral membrane protein exit from TGN. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008],
Specifications
| Size | 50μL/100μL |
| Target | PKD2 |
| Reactivity | Human, Mouse |
| Applications | WB, IHC, IF, ELISA |
| MW(Calculated) | 97kD |
| MW(Observed) | 115kD |
| Host Species | Rabbit |
| Isotype | IgG,Kappa |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | IHC 1:200-1:500; WB 1:2000-1:10000; IF 1:200-1:1000; ELISA 1:5000-1:20000; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Purification | Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | RM1685R |
| Immunogen | The specific immunogen used to produce this antibody is proprietary information. |
| Specificity | Endogenous |
| Gene Name | PRKD2 |
| Protein Name | Serine/threonine-protein kinase D2 |
| Other Name | PRKD2; / PKD2; / HSPC187; / Serine/threonine-protein kinase D2; / nPKC-D2 |
| Species | Human |
| Gene ID | 25865 |
| Uniprot | Q9BZL6, |
| Species.1 | Mouse |
| Gene ID.1 | 101540 |
| Uniprot.1 | Q8BZ03, |
| Species.2 | Rat |
| Gene ID.2 | 292658 |
| Uniprot.2 | Q5XIS9 |
| Cellular Localization | Cytoplasm . Cell membrane . Nucleus . Golgi apparatus, trans-Golgi network . Translocation to the cell membrane is required for kinase activation. Accumulates in the nucleus upon CK1-mediated phosphorylation after activation of G-protein-coupled receptors. Nuclear accumulation is regulated by blocking nuclear export of active PRKD2 rather than by increasing import. . |
| Tissue Expression | Widely expressed. |
| Signaling_pathway | Organismal Systems >> Endocrine system >> Aldosterone synthesis and secretion;Environmental Information Processing >> Signal transduction >> Rap1 signaling pathway |
| Research Areas | >>Rap1 signaling pathway; / >>Aldosterone synthesis and secretion; / >>Chemical carcinogenesis - reactive oxygen species |
| Function | Catalytic activity:ATP + a protein = ADP + a phosphoprotein.,enzyme regulation:Activated by diacylglycerol and phorbol esters.,Function:Calcium-independent, phospholipid-dependent, serine- and threonine-specific protein kinase.,PTM:Autophosphorylated. Phorbol esters stimulates autophosphorylation. Phosphorylation of Ser-876 correlates with the activation status of the kinase.,similarity:Belongs to the protein kinase superfamily.,similarity:Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. PKD subfamily.,similarity:Contains 1 PH domain.,similarity:Contains 1 protein kinase domain.,similarity:Contains 2 phorbol-ester/DAG-type zinc fingers.,tissue specificity:Widely expressed., |
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