Name: U-Blot® PMEL/Melanoma gp100 (HMB45) Mouse mAb (Ready to Use)
SKU: WM1754R
Price: 168 USD
Availability: InStock

Product details

Background：
This gene encodes a melanocyte-specific type I transmembrane glycoprotein. The encoded protein is enriched in melanosomes, which are the melanin-producing organelles in melanocytes, and plays an essential role in the structural organization of premelanosomes. This protein is involved in generating internal matrix fibers that define the transition from Stage I to Stage II melanosomes. This protein undergoes a complex pattern of prosttranslational processing and modification that is essential to the proper functioning of the protein. A secreted form of this protein that is released by proteolytic ectodomain shedding may be used as a melanoma-specific serum marker. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jan 2011],

Specifications

Target	HMB45
Reactivity	Human, Mouse
Applications	IHC
MW(Calculated)
MW(Observed)
Host Species	Mouse
Isotype	IgG1,Kappa
Conjugate/Modification	Unmodified
Modification
Recommended Dilution Ratio	Ready to use for IHC / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#YS0004)
Formulation	The prediluted ready-to-use antibody is diluted in phosphate buffer saline containing stabilizing protein and 0.05% Proclin 300
Source
Purification	The antibody was affinity-purified from ascites by affinity-chromatography using specific immunogen.
Purity
Storage	2°C to 8°C/1 year,Ship by ice bag
Concentration
Clonality	Monoclonal
Clone Number	ABT255
Immunogen	Synthesized peptide derived from human PMEL AA range: 400-500
Sequence
Specificity	The antibody can specifically recognize human PMEL protein.
Gene Name	PMEL D12S53E PMEL17 SILV
Protein Name	95 kDa melanocyte specific secreted glycoprotein; 95 kDa melanocyte-specific secreted glycoprotein; D12S53E; gp100; M-beta; ME20; ME20 M/ME20 S; ME20-M; ME20-S; ME20M; ME20M/ME20S; ME20S; Melanocyte lineage specific antigen GP100; Melanocyte protein mel 17; Melanocyte protein Pmel 17; Melanocyte protein Pmel 17 precursor; Melanocytes lineage-specific antigen GP100; Melanoma associated ME20 antigen; Melanoma gp100; Melanoma-associated ME20 antigen; Melanosomal matrix protein 17; Melanosomal matrix protein17; P1; p100; p26; PMEL 17; PMEL; PMEL_HUMAN; PMEL17; Premelanosome protein; Secreted melanoma-associated ME20 antigen; SI; SIL; SILV; Silver (mouse homolog) like; Silver homolog; Silver locus protein homolog; Silver, mouse, homolog of
Other Name	95 kDa melanocyte specific secreted glycoprotein; / 95 kDa melanocyte-specific secreted glycoprotein; / D12S53E; / gp100; / M-beta; / ME20; / ME20 M/ME20 S; / ME20-M; / ME20-S; / ME20M; / ME20M/ME20S; / ME20S; / Melanocyte lineage specific antigen GP100; / Melanocyte protein mel 17; / Melanocyte protein Pmel 17; / Melanocyte protein Pmel 17 precursor; / Melanocytes lineage-specific antigen GP100; / Melanoma associated ME20 antigen; / Melanoma gp100; / Melanoma-associated ME20 antigen; / Melanosomal matrix protein 17; / Melanosomal matrix protein17; / P1; / p100; / p26; / PMEL 17; / PMEL; / PMEL_HUMAN; / PMEL17; / Premelanosome protein; / Secreted melanoma-associated ME20 antigen; / SI; / SIL; / SILV; / Silver; / mouse homolog; / like; / Silver homolog; / Silver locus protein homolog; / HMB45
Species	Human
Gene ID-1
Uniprot	P40967,
Species.1	Mouse
Gene ID-2
Uniprot.1	Q60696
Species.2
Gene ID-3
Uniprot.2
Organism-4
Gene ID-4
SwissProt-4
Background	This gene encodes a melanocyte-specific type I transmembrane glycoprotein. The encoded protein is enriched in melanosomes, which are the melanin-producing organelles in melanocytes, and plays an essential role in the structural organization of premelanosomes. This protein is involved in generating internal matrix fibers that define the transition from Stage I to Stage II melanosomes. This protein undergoes a complex pattern of prosttranslational processing and modification that is essential to the proper functioning of the protein. A secreted form of this protein that is released by proteolytic ectodomain shedding may be used as a melanoma-specific serum marker. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jan 2011],
Cellular Localization	Cytoplasmic
Tissue Expression	Skin/ Melanoma
Signaling_pathway
Research Areas
Function	Function:Could be a melanogenic enzyme. Could represent an oncofetal self-antigen that is normally expressed at low levels in quiescent adult melanocytes but overexpressed by proliferating neonatal melanocytes and during tumor growth. Release of the soluble form, ME20-S, could protect tumor cells from antibody mediated immunity.,similarity:Belongs to the Pmel-17/NMB family.,similarity:Contains 1 PKD domain.,subcellular location:Identified by mass spectrometry in melanosome fractions from stage I to stage IV.,subcellular location:Probably product of proteolytic cleavage.,tissue specificity:Preferentially expressed in melanomas. Some expression was found in dysplastic nevi. Not found in normal tissues nor in carcinomas.,
RRID

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U-Blot® PMEL/Melanoma gp100 (HMB45) Mouse mAb (Ready to Use)

Product details

Specifications

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U-Blot® PMEL/Melanoma gp100 (HMB45) Mouse mAb (Ready to Use)

Product details

Specifications

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