Name: U-Blot® PPP3CA Rabbit mAb
SKU: WR5224
Price: 268 USD
Availability: InStock

Product details

Background:catalytic activity:A phosphoprotein + H(2)O = a protein + phosphate.,cofactor:Binds 1 Fe(3+) ion per subunit.,cofactor:Binds 1 zinc ion per subunit.,function:Calcium-dependent, calmodulin-stimulated protein phosphatase. This subunit may have a role in the calmodulin activation of calcineurin. Dephosphorylates HSPB1 and SSH1.,online information:Calcineurin entry,similarity:Belongs to the PPP phosphatase family.,similarity:Belongs to the PPP phosphatase family. PP-2B subfamily.,subcellular location:Colocalizes with ACTN1 and MYOZ2 at the Z line in heart and skeletal muscle.,subunit:Composed of two components (A and B), the A component is the catalytic subunit and the B component confers calcium sensitivity. Interacts with TORC2/CRTC2, MYOZ1, MYOZ2 and MYOZ3.,

Specifications

Size	50μL/100μL
Target	PPP3CA
Reactivity	Human, Mouse, Rat
Applications	WB, IHC, IF, IP, ELISA
MW(Calculated)	59kD
MW(Observed)	32,59kD
Host Species	Rabbit
Isotype	IgG,Kappa
Conjugate/Modification	Unmodified
Recommended Dilution Ratio	IHC 1:200-1:1000; WB 1:2000-1:10000; IF 1:200-1:1000; ELISA 1:5000-1:20000; IP 1:50-1:200; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004)
Formulation	PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
Purification	Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography.
Storage	-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration
Clonality	Monoclonal
Clone Number	RM1461R
Immunogen	The specific immunogen used to produce this antibody is proprietary information.
Specificity	Endogenous
Gene Name	PPP3CA CALNA CNA
Protein Name	PP2B-Aα
Other Name	PP2B-Aα; / PPP3CA; / CALNA; / CNA; / Serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform; / CAM-PRP catalytic subunit; / Calmodulin-dependent calcineurin A subunit alpha isoform
Species	Human
Gene ID	5530
Uniprot	Q08209,
Species.1	Mouse
Gene ID.1	19055
Uniprot.1	P63328,
Species.2	Rat
Gene ID.2	24674
Uniprot.2	P63329
Cellular Localization	Cytoplasm . Cell membrane; Peripheral membrane protein . Cell membrane, sarcolemma . Cytoplasm, myofibril, sarcomere, Z line . Cell projection, dendritic spine . Colocalizes with ACTN1 and MYOZ2 at the Z line in heart and skeletal muscle (By similarity). Recruited to the cell membrane by scaffold protein AKAP5 following L-type Ca(2+)-channel activation (PubMed:22343722). .
Tissue Expression	Expressed in keratinocytes (at protein level) (PubMed:29043977). Expressed in lymphoblasts (at protein level) (PubMed:30254215).
Signaling_pathway	Cellular Processes >> Cell growth and death >> Cellular senescence;Organismal Systems >> Immune system >> Natural killer cell mediated cytotoxicity;Organismal Systems >> Immune system >> T cell receptor signaling pathway;Organismal Systems >> Immune system >> Th1 and Th2 cell differentiation;Organismal Systems >> Immune system >> Th17 cell differentiation;Organismal Systems >> Immune system >> B cell receptor signaling pathway;Organismal Systems >> Endocrine system >> Glucagon signaling pathway;Organismal Systems >> Endocrine system >> Oxytocin signaling pathway;Organismal Systems >> Nervous system >> Glutamatergic synapse;Organismal Systems >> Nervous system >> Dopaminergic synapse;Organismal Systems >> Development and regeneration >> Osteoclast differentiation;Human Diseases >> Cancer: overview >> PD-L1 expression and PD-1 checkpoint pathway in cancer;Human Diseases >> Neurodegenerative disease >> Alzheimer disease;Human Diseases >> Neurodegenerative disease >> Amyotrophic lateral sclerosis;Human Diseases >> Neurodegenerative disease >> Prion disease;Human Diseases >> Neurodegenerative disease >> Pathways of neurodegeneration - multiple diseases;Environmental Information Processing >> Signal transduction >> MAPK signaling pathway;Environmental Information Processing >> Signal transduction >> Wnt signaling pathway;Environmental Information Processing >> Signal transduction >> VEGF signaling pathway;Environmental Information Processing >> Signal transduction >> Calcium signaling pathway;Environmental Information Processing >> Signal transduction >> cGMP-PKG signaling pathway
Research Areas	>>MAPK signaling pathway; / >>Calcium signaling pathway; / >>cGMP-PKG signaling pathway; / >>Oocyte meiosis; / >>Cellular senescence; / >>Wnt signaling pathway; / >>Axon guidance; / >>VEGF signaling pathway; / >>Osteoclast differentiation; / >>C-type lectin receptor signaling pathway; / >>Natural killer cell mediated cytotoxicity; / >>Th1 and Th2 cell differentiation; / >>Th17 cell differentiation; / >>T cell receptor signaling pathway; / >>B cell receptor signaling pathway; / >>Long-term potentiation; / >>Glutamatergic synapse; / >>Dopaminergic synapse; / >>Oxytocin signaling pathway; / >>Glucagon signaling pathway; / >>Renin secretion; / >>Alzheimer disease; / >>Amyotrophic lateral sclerosis; / >>Prion disease; / >>Pathways of neurodegeneration - multiple diseases; / >>Amphetamine addiction; / >>Tuberculosis; / >>Human cytomegalovirus infection; / >>Human T-cell leukemia virus 1 infection; / >>Kaposi sarcoma-associated herpesvirus infection; / >>Human immunodeficiency virus 1 infection; / >>PD-L1 expression and PD-1 checkpoint pathway in cancer; / >>Lipid and atherosclerosis
Function	Catalytic activity:A phosphoprotein + H(2)O = a protein + phosphate.,cofactor:Binds 1 Fe(3+) ion per subunit.,cofactor:Binds 1 zinc ion per subunit.,Function:Calcium-dependent, calmodulin-stimulated protein phosphatase. This subunit may have a role in the calmodulin activation of calcineurin. Dephosphorylates HSPB1 and SSH1.,online information:Calcineurin entry,similarity:Belongs to the PPP phosphatase family.,similarity:Belongs to the PPP phosphatase family. PP-2B subfamily.,subcellular location:Colocalizes with ACTN1 and MYOZ2 at the Z line in heart and skeletal muscle.,subunit:Composed of two components (A and B), the A component is the catalytic subunit and the B component confers calcium sensitivity. Interacts with TORC2/CRTC2, MYOZ1, MYOZ2 and MYOZ3.,