U-Blot® PRKAR2A Rabbit mAb
Ucallm_WR5384-50
Price range: $268.00 through $328.00
Product details
Background:protein kinase cAMP-dependent type II regulatory subunit alpha(PRKAR2A) Homo sapiens cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. The protein encoded by this gene is one of the regulatory subunits. This subunit can be phosphorylated by the activated catalytic subunit. It may interact with various A-kinase anchoring proteins and determine the subcellular localization of cAMP-dependent protein kinase. This subunit has b
Specifications
| Size | 50μL/100μL |
| Target | PRKAR2A |
| Reactivity | Human |
| Applications | WB, IHC, IF, IP, ELISA |
| MW(Calculated) | 46kD |
| MW(Observed) | 54kD |
| Host Species | Rabbit |
| Isotype | IgG,Kappa |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | IHC 1:1000-1:2000; WB 1:2000-1:10000; IF 1:200-1:1000; ELISA 1:5000-1:20000; IP 1:50-1:200; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Purification | Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | RM1628R |
| Immunogen | The specific immunogen used to produce this antibody is proprietary information. |
| Specificity | Endogenous |
| Gene Name | PRKAR2A |
| Protein Name | cAMP-dependent protein kinase type II-alpha regulatory subunit |
| Other Name | PRKAR2A; / PKR2; / PRKAR2; / cAMP-dependent protein kinase type II-alpha regulatory subunit |
| Species | Human |
| Gene ID | 5576 |
| Uniprot | P13861, |
| Species.1 | Mouse |
| Gene ID.1 | |
| Uniprot.1 | P12367 |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | Cytoplasm . Cell membrane . Colocalizes with PJA2 in the cytoplasm and the cell membrane. |
| Tissue Expression | Four types of regulatory chains are found: I-alpha, I-beta, II-alpha, and II-beta. Their expression varies among tissues and is in some cases constitutive and in others inducible. |
| Signaling_pathway | Organismal Systems >> Endocrine system >> Insulin signaling pathway |
| Research Areas | >>Insulin signaling pathway |
| Function | Function:Type II regulatory chains mediate membrane association by binding to anchoring proteins, including the MAP2 kinase.,PTM:Phosphorylated by the activated catalytic chain.,similarity:Belongs to the cAMP-dependent kinase regulatory chain family.,similarity:Contains 2 cyclic nucleotide-binding domains.,subunit:The inactive form of the enzyme is composed of two regulatory chains and two catalytic chains. Activation by cAMP produces two active catalytic monomers and a regulatory dimer that binds four cAMP molecules. Interacts with AKAP4 and CBFA2T3.,tissue specificity:Four types of regulatory chains are found: I-alpha, I-beta, II-alpha, and II-beta. Their expression varies among tissues and is in some cases constitutive and in others inducible., |
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