Price range: $268.00 through $328.00
Background：
This gene encodes a member of the profilin family of small actin-binding proteins. The encoded protein plays an important role in actin dynamics by regulating actin polymerization in response to extracellular signals. Deletion of this gene is associated with Miller-Dieker syndrome , and the encoded protein may also play a role in Huntington disease. Multiple pseudogenes of this gene are located on chromosome 1. [provided by RefSeq , Jul 2012] ,
| Size | 50μL/100μL |
| Target | PROF1 |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC, IF, IP, ELISA |
| MW(Calculated) | 15kD |
| MW(Observed) | 15kD |
| Host Species | Rabbit |
| Isotype | IgG, Kappa |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | IHC 1:200-1:1000; WB 1:2000-1:10000; IF 1:200-1:1000; ELISA 1:5000-1:20000; IP 1:50-1:200 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Purification | Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography. |
| Storage | -15°C to -25°C/1 year (Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | RM1994R |
| Immunogen | The specific immunogen used to produce this antibody is proprietary information. |
| Specificity | Endogenous |
| Gene Name | PFN1 |
| Protein Name | Profilin-1 (Epididymis tissue protein Li 184a) (Profilin I) |
| Other Name | |
| Species | Human |
| Gene ID | 5216 |
| Uniprot | P07737, |
| Species.1 | Mouse |
| Gene ID.1 | |
| Uniprot.1 | P62962, |
| Species.2 | Rat |
| Gene ID.2 | |
| Uniprot.2 | P62963 |
| Cellular Localization | Cytoplasm , cytoskeleton. |
| Tissue Expression | Expressed in epididymis (at protein level) . |
| Signaling_pathway | Cellular Processes >> Cell motility >> Regulation of actin cytoskeleton;Human Diseases >> Neurodegenerative disease >> Amyotrophic lateral sclerosis;Environmental Information Processing >> Signal transduction >> Rap1 signaling pathway |
| Research Areas | >>Rap1 signaling pathway; / >>Regulation of actin cytoskeleton; / >>Amyotrophic lateral sclerosis; / >>Shigellosis; / >>Salmonella infection |
| Function | Function:Binds to actin and affects the structure of the cytoskeleton. At high concentrations , profilin prevents the polymerization of actin , whereas it enhances it at low concentrations. By binding to PIP2 , it inhibits the formation of IP3 and DG. ,similarity:Belongs to the profilin family. ,subunit:Occurs in many kinds of cells as a complex with monomeric actin in a 1:1 ratio. Found in a complex with XPO6 , Ran , ACTB and PFN1. , |
