Name: U-Blot® Prothrombin Rabbit mAb
SKU: WR6065
Price: 268 USD
Availability: InStock

Product details

Background：
Coagulation factor II is proteolytically cleaved to form thrombin in the first step of the coagulation cascade which ultimately results in the stemming of blood loss. F2 also plays a role in maintaining vascular integrity during development and postnatal life. Peptides derived from the C-terminus of this protein have antimicrobial activity against E. coli and P. aeruginosa. Mutations in F2 lead to various forms of thrombosis and dysprothrombinemia. Alternative splicing results in multiple transcript variants. [provided by RefSeq , Aug 2015] ,

Specifications

Size	50μL/100μL
Target	Prothrombin
Reactivity	Mouse, Rat
Applications	WB, IHC, IF, ELISA
MW(Calculated)	70kD
MW(Observed)	85kD
Host Species	Rabbit
Isotype	IgG,Kappa
Conjugate/Modification	Unmodified
Recommended Dilution Ratio	IHC 1:200-1:1000; WB 1:2000-1:10000; IF 1:200-1:1000; ELISA 1:5000-1:20000; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004)
Formulation	PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
Purification	Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography.
Storage	-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration
Clonality	Monoclonal
Clone Number	RM1977R
Immunogen	The specific immunogen used to produce this antibody is proprietary information.
Specificity	Endogenous
Gene Name	F2
Protein Name	Prothrombin
Other Name	F2; / Prothrombin; / Coagulation factor II
Species	Human
Gene ID	2147
Uniprot	P00734,
Species.1	Mouse
Gene ID.1	14061
Uniprot.1	P19221,
Species.2	Rat
Gene ID.2
Uniprot.2	P18292
Cellular Localization	Secreted, extracellular space.
Tissue Expression	Expressed by the liver and secreted in plasma.
Signaling_pathway	Cellular Processes >> Cell motility >> Regulation of actin cytoskeleton;Organismal Systems >> Immune system >> Complement and coagulation cascades;Organismal Systems >> Immune system >> Platelet activation;Human Diseases >> Cancer: overview >> Pathways in cancer;Environmental Information Processing >> Signal transduction >> Phospholipase D signaling pathway
Research Areas	>>Phospholipase D signaling pathway; / >>Neuroactive ligand-receptor interaction; / >>Complement and coagulation cascades; / >>Platelet activation; / >>Regulation of actin cytoskeleton; / >>Pathogenic Escherichia coli infection; / >>Coronavirus disease - COVID-19; / >>Pathways in cancer
Function	Catalytic activity:Selective cleavage of Arg-\|-Gly bonds in fibrinogen to form fibrin and release fibrinopeptides A and B.,Disease:Defects in F2 are the cause of various forms of dysprothrombinemia [MIM:176930].,Disease:Genetic variations in F2 may be a cause of susceptibility to ischemic stroke [MIM:601367]; also known as cerebrovascular accident or cerebral infarction. A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors.,Function:Thrombin, which cleaves bonds after Arg and Lys, converts fibrinogen to fibrin and activates factors V, VII, VIII, XIII, and, in complex with thrombomodulin, protein C. Functions in blood homeostasis, inflammation and wound healing.,miscellaneous:It is not known whether 1 or 2 smaller activation peptides, with additional cleavage after Arg-314, are released in natural blood clotting.,miscellaneous:Prothrombin is activated on the surface of a phospholipid membrane that binds the amino end of prothrombin and factors Va and Xa in Ca-dependent interactions; factor Xa removes the activation peptide and cleaves the remaining part into light and heavy chains. The activation process starts slowly because factor V itself has to be activated by the initial, small amounts of thrombin.,miscellaneous:The cleavage after Arg-198, observed in vitro, does not occur in plasma.,miscellaneous:Thrombin can itself cleave the N-terminal fragment (fragment 1) of the prothrombin, prior to its activation by factor Xa.,online information:Thrombin entry,pharmaceutical:The peptide TP508 also known as Chrysalin (Orthologic) could be used to accelerate repair of both soft and hard tissues.,PTM:The gamma-carboxyglutamyl residues, which bind calcium ions, result from the carboxylation of glutamyl residues by a microsomal enzyme, the vitamin K-dependent carboxylase. The modified residues are necessary for the calcium-dependent interaction with a negatively charged phospholipid surface, which is essential for the conversion of prothrombin to thrombin.,similarity:Belongs to the peptidase S1 family.,similarity:Contains 1 Gla (gamma-carboxy-glutamate) domain.,similarity:Contains 1 peptidase S1 domain.,similarity:Contains 2 kringle domains.,tissue specificity:Expressed by the liver and secreted in plasma.,

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U-Blot® Prothrombin Rabbit mAb

Product details

Specifications

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U-Blot® Prothrombin Rabbit mAb

Product details

Specifications

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