U-Blot® RNA pol II CTD monomethyl Lys7 Monoclonal Antibody
UcallM
$999.00
Product details
Background:SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 2 (SMARCA2) , also known as BRM , is a member of the SWI/SNF family of ATP-dependant chromatin remodeling proteins. SMARCA2 is the catalytic subunit of a multi-subunit protein complex that utilizes the energy from ATP hydrolysis to alter chromatin structure and activate transcription. It is thought that PROTACS (Proteolysis Targeting Chimeras) as degraders of SMARCA4 (BRG1) and SMARCA2 (BRM) may offer a strategy for research associated with treatment or prevention of cancer by modulation of SMARCA2/4.
Specifications
| Target | -- |
| Reactivity | Human |
| Applications | ChIP; ChIP-Seq |
| MW(Calculated) | 240 kDa |
| Host Species | Rat |
| Isotype | IgG2a |
| Recommended Dilution Ratio | Applications Validated by Active Motif: ChIP-Seq: 10 µg per ChIP IP: 5 µg per IP WB*: 0.5 - 2 µg/ml *Note: Many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western blot. |
| Formulation | PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic. |
| Purification | Protein G Chromatography |
| Storage | Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage. |
| Concentration | -- |
| Clonality | Monoclonal |
| Clone Number | 1F5 |
| Immunogen | This antibody was raised against a peptide containing dimethyl-lysine 7 of human RNA Pol II CTD. |
| Specificity | -- |
| Gene Name | -- |
| Other Name | RNA Polymerase II Carboxy Terminal Domain |
| Gene ID | -- |
| Cellular Localization | -- |
| Function | -- |
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