Price range: $268.00 through $328.00
Background：
This gene encodes a microsomal protein expressed at high levels in androgen-sensitive tissues such as the prostate. The encoded protein is active at acidic pH and is sensitive to the 4-azasteroid inhibitor finasteride. Deficiencies in this gene can result in male pseudohermaphroditism , specifically pseudovaginal perineoscrotal hypospadias (PPSH) . [provided by RefSeq , Jul 2008] ,
| Size | 50μL/100μL |
| Target | S5A2 |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC, IF, IP, ELISA |
| MW(Calculated) | 28kD |
| MW(Observed) | 28kD |
| Host Species | Rabbit |
| Isotype | IgG,Kappa |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | IHC 1:200-1:1000; WB 1:2000-1:10000; IF 1:200-1:1000; ELISA 1:5000-1:20000; IP 1:50-1:200; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Purification | Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | RM1957R |
| Immunogen | The specific immunogen used to produce this antibody is proprietary information. |
| Specificity | Endogenous |
| Gene Name | SRD5A2 |
| Protein Name | 3-oxo-5-alpha-steroid 4-dehydrogenase 2 (5 alpha-SR2) (SR type 2) (Steroid 5-alpha-reductase 2) (S5AR 2) (Type II 5-alpha reductase) |
| Other Name | |
| Species | Human |
| Gene ID | 6716 |
| Uniprot | P31213, |
| Species.1 | Mouse |
| Gene ID.1 | |
| Uniprot.1 | Q99N99, |
| Species.2 | Rat |
| Gene ID.2 | |
| Uniprot.2 | P31214 |
| Cellular Localization | Microsome membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein . |
| Tissue Expression | Expressed in high levels in the prostate and many other androgen-sensitive tissues. |
| Signaling_pathway | Human Diseases >> Cancer: specific types >> Prostate cancer |
| Research Areas | >>Steroid hormone biosynthesis; / >>Metabolic pathways; / >>Prostate cancer |
| Function | Catalytic activity:A 3-oxo-5-alpha-steroid + acceptor = a 3-oxo-Delta(4)-steroid + reduced acceptor.,Disease:Defects in SRD5A2 are the cause of pseudovaginal perineoscrotal hypospadias (PPSH) [MIM:264600]; also known as male pseudohermaphroditism due to 5-alpha-reductase deficiency or 5-ARD deficiency. PPSH is a rare form of male pseudohermaphroditism in which affected males develop normal internal urogenital tracts but fail to develop external male structures. Individuals with PPSH have testicles and tend to have a vagina and labia, but with a small penis capable of ejaculation instead of a clitoris (this penis, however, appears to be a clitoris at birth). These individuals are normally raised as girls. However at puberty their testes will descend, their voice will deepen and they often will develop a male sexual identity. They develop only limited facial hair and will not experience male-pattern baldness. Jeffrey Eugenides won a Pulitzer prize for his 2003 novel 'Middlesex' which describes the life an individual with such a deficiency.,Function:Converts testosterone (T) into 5-alpha-dihydrotestosterone (DHT) and progesterone or corticosterone into their corresponding 5-alpha-3-oxosteroids. It plays a central role in sexual differentiation and androgen physiology.,online information:5-alpha reductase entry,polymorphism:Individuals with Thr-49 have an increased risk of prostate cancer. The enzyme with Thr-49 has a higher in vitro V(max) than the Ala-49 enzyme.,similarity:Belongs to the steroid 5-alpha reductase family.,tissue specificity:Expressed in high levels in the prostate and many other androgen-sensitive tissues., |
