IHC 1:200-1:1000; WB 1:2000-1:10000; IF 1:200-1:1000; ELISA 1:5000-1:20000; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004)
Formulation
PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
Purification
Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography.
Storage
-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration
Clonality
Monoclonal
Clone Number
RM1932R
Immunogen
The specific immunogen used to produce this antibody is proprietary information.
Specificity
Endogenous
Gene Name
SH3GLB1 KIAA0491 CGI-61
Protein Name
Endophilin-B1 (Bax-interacting factor 1) (Bif-1) (SH3 domain-containing GRB2-like protein B1)
Other Name
Species
Human
Gene ID
51100
Uniprot
Q9Y371,
Species.1
Mouse
Gene ID.1
54673
Uniprot.1
Q9JK48,
Species.2
Rat
Gene ID.2
54673
Uniprot.2
Q6AYE2
Cellular Localization
Cytoplasm . Golgi apparatus membrane; Peripheral membrane protein . Mitochondrion outer membrane; Peripheral membrane protein . Cytoplasmic vesicle, autophagosome membrane . Midbody . Association with the Golgi apparatus depends on the cell type (By similarity). Following starvation colocalizes with ATG5 and LC3 autophagy-related protein(s)on autophagosomal membranes (PubMed:17891140). .
Tissue Expression
Highly expressed in heart, skeletal muscle, kidney and placenta. Detected at lower levels in brain, colon, thymus, spleen, liver, small intestine, lung and peripheral blood leukocytes.
Signaling_pathway
Cellular Processes >> Transport and catabolism >> Autophagy - animal