U-Blot® Smad2 (phospho S255) Rabbit mAb
Ucallm_WT9109-100
Price range: $288.00 through $368.00
Product details
Background:SMAD2, also named as MADH2 and MADR2, belongs to the dwarfin/SMAD family, contains 1 MH1 (MAD homology 1) domain and 1 MH2 (MAD homology 2) domain. SMAD2 is a receptor-regulated SMAD(R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. This protein may act as a tumor suppressor in colorectal carcinoma. It is phosphorylated on one or several of Thr-220, Ser-245, Ser-250, and Ser-255. In response to TGF-beta, It is phosphorylated on Ser-465/467 by TGF-beta and activin type 1 receptor kinases, and then able to interact with SMURF2, recruiting other proteins, such as SNON, for degradation. In response to decorin, the naturally occurring inhibitor of TGF-beta signaling, it is phosphorylated on Ser-240 by CaMK2. It is phosphorylated by MAPK3 upon EGF stimulation; which increases transcriptional activity and stability, and is blocked by calmodulin.
Specifications
| Target | Phospho-Smad2 (Ser255) |
| Reactivity | Human |
| Application | WB |
| MW(Calculated) | 52 kDa |
| MW(Observed) | 58 kDa |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate | Unconjugated |
| Modification | Phosphorylation |
| Modification Site | Ser255 |
| Recommended dilution ratio | WB 1:1000 |
| Composition | PBS, Glycerol, BSA |
| Purification process | Protein A affinity purification |
| Storage | Store at -20°C. Avoid repeated freeze-thaw cycles |
| Stability | Stable for 12 months from date of receipt / reconstitution. |
| Concentration | |
| Clonal | Recombinant monoclonal |
| Clone Number | W-R77 |
| Immunogen | polypeptide |
| Gene Name | SMAD2 |
| Protein Name | Mothers against decapentaplegic homolog 2 |
| Alternative name | MADH2, MADR2 |
| Organism-1 | Human |
| Gene ID | 4087 |
| SwissProt-1 | Q15796 |
| Cellular Localization | Cytoplasm,Nucleus |
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