SKU: WR5953 Categories: ,

U-Blot® TIA1 Rabbit mAb

Price range: $268.00 through $328.00

SKU: WR5953-50
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Product details

Background:
The product encoded by this gene is a member of a RNA-binding protein family and possesses nucleolytic activity against cytotoxic lymphocyte (CTL) target cells. It has been suggested that this protein may be involved in the induction of apoptosis as it preferentially recognizes poly (A) homopolymers and induces DNA fragmentation in CTL targets. The major granule-associated species is a 15-kDa protein that is thought to be derived from the carboxyl terminus of the 40-kDa product by proteolytic processing. Alternative splicing resulting in different isoforms of this gene product has been described in the literature. [provided by RefSeq , Jul 2008] ,

Specifications

Size50μL/100μL
TargetTIA1
ReactivityHuman, Mouse, Rat
ApplicationsWB, IHC, IF, IP, ELISA
MW(Calculated)43kD
MW(Observed)42,44kD
Host SpeciesRabbit
IsotypeIgG,Kappa
Conjugate/ModificationUnmodified
Recommended Dilution RatioIHC 1:200-1:1000; WB 1:2000-1:10000; IF 1:200-1:1000; ELISA 1:5000-1:20000; IP 1:50-1:200; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004)
FormulationPBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
PurificationRecombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography.
Storage-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration
ClonalityMonoclonal
Clone NumberRM1891R
ImmunogenThe specific immunogen used to produce this antibody is proprietary information.
SpecificityEndogenous
Gene NameTIA1
Protein NameNucleolysin TIA-1 isoform p40 (RNA-binding protein TIA-1) (T-cell-restricted intracellular antigen-1) (TIA-1) (p40-TIA-1)
Other Name
SpeciesHuman
Gene ID7072
UniprotP31483,
Species.1Mouse
Gene ID.1
Uniprot.1P52912
Species.2
Gene ID.2
Uniprot.2
Cellular LocalizationNucleus . Cytoplasm . Cytoplasm, Stress granule . Accumulates in cytoplasmic stress granules (SG) following cellular damage (PubMed:15371533, PubMed:10613902). Recruitment to SG is induced by Zn(2+) (By similarity). .
Tissue ExpressionExpressed in heart, small intestine, kidney, liver, lung, skeletal muscle, testes, pancreas, and ovary (at protein level).
Signaling_pathway
Research Areas
FunctionFunction:RNA-binding protein. Possesses nucleolytic activity against cytotoxic lymphocyte target cells. May be involved in apoptosis.,PTM:TIA-1 major granule-associated species is a 15-kDa protein (p15-TIA-1) that seems to be derived by proteolytic processing from the C-terminus of p40-TIA-1.,similarity:Contains 3 RRM (RNA recognition motif) domains.,subcellular location:Cytoplasmic granules of cytolytic T-lymphocytes.,
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