U-Blot® TIF1α Rabbit mAb
Ucallm_WR6031-50
Price range: $268.00 through $328.00
Product details
Background：
The protein encoded by this gene mediates transcriptional control by interaction with the activation function 2 (AF2) region of several nuclear receptors , including the estrogen , retinoic acid , and vitamin D3 receptors. The protein localizes to nuclear bodies and is thought to associate with chromatin and heterochromatin-associated factors. The protein is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains – a RING , a B-box type 1 and a B-box type 2 – and a coiled-coil region. Two alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq , Jul 2008] ,
Specifications
| Size | 50μL/100μL |
| Target | TIF1α |
| Reactivity | Human, Mouse |
| Applications | WB, IHC, IF, ELISA |
| MW(Calculated) | 117kD |
| MW(Observed) | 125kD |
| Host Species | Rabbit |
| Isotype | IgG,Kappa |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | IHC 1:200-1:1000; WB 1:2000-1:10000; IF 1:200-1:1000; ELISA 1:5000-1:20000; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Purification | Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | RM1928R |
| Immunogen | The specific immunogen used to produce this antibody is proprietary information. |
| Specificity | Endogenous |
| Gene Name | TRIM24 |
| Protein Name | Transcription intermediary factor 1-alpha |
| Other Name | TRIM24; / RNF82; / TIF1; / TIF1A; / Transcription intermediary factor 1-alpha; / TIF1-alpha; / E3 ubiquitin-protein ligase TRIM24; / RING finger protein 82; / Tripartite motif-containing protein 24 |
| Species | Human |
| Gene ID | 8805 |
| Uniprot | O15164, |
| Species.1 | Mouse |
| Gene ID.1 | 21848 |
| Uniprot.1 | Q64127 |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | Nucleus . Cytoplasm . Mitochondrion . Colocalizes with sites of active transcription. Detected both in nucleus and cytoplasm in some breast cancer samples. Predominantly nuclear. Translocated from nucleus to mitochondria to mediate antiviral immunity (PubMed:32324863). . |
| Tissue Expression | Brain,Epithelium,Mammary cancer,Testis,Thyroid, |
| Signaling_pathway | |
| Research Areas | |
| Function | Disease:A chromosomal aberration involving TIF1 is a cause of thyroid papillary carcinoma (PACT) [MIM:188550]. Translocation t(7; 10)(q32; q11) with RET. The translocation generates the TIF1/RET (PTC6) oncogene.,Function:Interacts selectively in vitro with the AF2-activating domain of the estrogen receptors. Association with DNA-bound estrogen receptors requires the presence of estradiol.,PTM:Phosphorylated upon DNA damage, probably by ATM or ATR.,similarity:Contains 1 bromo domain.,similarity:Contains 1 PHD-type zinc finger.,similarity:Contains 1 RING-type zinc finger.,similarity:Contains 2 B box-type zinc fingers.,subunit:Interacts with CBX1 and CBX3 (By similarity). Interacts with NR3C2., |
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