Name: U-Blot® TLR5 Rabbit mAb
SKU: WR5088
Price: 268 USD
Availability: InStock

Product details

Background:This gene encodes a member of the toll-like receptor (TLR) family, which plays a fundamental role in pathogen recognition and activation of innate immune responses. These receptors recognize distinct pathogen-associated molecular patterns that are expressed on infectious agents. The protein encoded by this gene recognizes bacterial flagellin, the principal component of bacterial flagella and a virulence factor. The activation of this receptor mobilizes the nuclear factor NF-kappaB, which in turn activates a host of inflammatory-related target genes. Mutations in this gene have been associated with both resistance and susceptibility to systemic lupus erythematosus, and susceptibility to Legionnaire disease.[provided by RefSeq, Dec 2009],

Specifications

Size	50μL/100μL
Target	TLR5
Reactivity	Human, Mouse, Rat
Applications	WB, IHC, IF, ELISA
MW(Calculated)	98kD
MW(Observed)	98kD
Host Species	Rabbit
Isotype	IgG,Kappa
Conjugate/Modification	Unmodified
Recommended Dilution Ratio	IHC 1:200-1:1000; WB 1:2000-1:10000; IF 1:200-1:1000; ELISA 1:5000-1:20000; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004)
Formulation	PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
Purification	Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography.
Storage	-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration
Clonality	Monoclonal
Clone Number	RM1340R
Immunogen	The specific immunogen used to produce this antibody is proprietary information.
Specificity	Endogenous
Gene Name	TLR5 TIL3
Protein Name	Toll-like receptor 5 (Toll/interleukin-1 receptor-like protein 3)
Other Name
Species	Human
Gene ID	7100
Uniprot	O60602,
Species.1	Mouse
Gene ID.1
Uniprot.1	Q9JLF7
Species.2
Gene ID.2
Uniprot.2
Cellular Localization	Cell membrane; Single-pass type I membrane protein .
Tissue Expression	Highly expressed on the basolateral surface of intestinal epithelia (PubMed:11489966). Expressed also in other cells such as lung epithelial cells (PubMed:11489966, PubMed:18490781).
Signaling_pathway	Organismal Systems >> Immune system >> Toll-like receptor signaling pathway;Human Diseases >> Immune disease >> Inflammatory bowel disease
Research Areas	>>Toll-like receptor signaling pathway; / >>Pathogenic Escherichia coli infection; / >>Shigellosis; / >>Salmonella infection; / >>Legionellosis; / >>Inflammatory bowel disease
Function	Disease:Genetic variation in TLR5 is associated with resistance to systemic lupus erythematosus type 1 (SLEB1) [MIM:601744]. Systemic lupus erythematosus (SLE) is a chronic autoimmune disease with a complex genetic basis. SLE is an inflammatory, and often febrile multisystemic disorder of connective tissue characterized principally by involvement of the skin, joints, kidneys, and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system.,Function:Participates in the innate immune response to microbial agents. Mediates detection of bacterial flagellins. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response.,polymorphism:Individuals with a common stop codon polymorphism in position 392 are unable to mediate flagellin signaling. This polymorphism acts in a dominant fashion and is associated with susceptibility to pneumonia caused by Legionella pneumophila [MIM:608556]. It also provides protection against systemic lupus erythematosus.,similarity:Belongs to the Toll-like receptor family.,similarity:Contains 1 TIR domain.,similarity:Contains 15 LRR (leucine-rich) repeats.,subunit:Binds MYD88 via their respective TIR domains.,tissue specificity:Highly expressed in ovary and in peripheral blood leukocytes, especially in monocytes, less in CD11c+ immature dendritic cells. Also detected in prostate and testis.,