U-Blot® UBC9 Rabbit mAb
Ucallm_WR5847-50
Price range: $268.00 through $328.00
Specifications
| Size | 50μL/100μL |
| Target | UBC9 |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC, IF, IP, ELISA |
| MW(Calculated) | 18kD |
| MW(Observed) | 18kD |
| Host Species | Rabbit |
| Isotype | IgG,Kappa |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | IHC 1:200-1:1000; WB 1:2000-1:10000; IF 1:200-1:1000; ELISA 1:5000-1:20000; IP 1:50-1:200; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Purification | Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | RM1816R |
| Immunogen | The specific immunogen used to produce this antibody is proprietary information. |
| Specificity | Endogenous |
| Gene Name | UBE2I UBC9 UBCE9 |
| Protein Name | SUMO-conjugating enzyme UBC9 |
| Other Name | UBE2I; / UBC9; / UBCE9; / SUMO-conjugating enzyme UBC9; / SUMO-protein ligase; / Ubiquitin carrier protein 9; / Ubiquitin carrier protein I; / Ubiquitin-conjugating enzyme E2 I; / Ubiquitin-protein ligase I; / p18 |
| Species | Human |
| Gene ID | 7329 |
| Uniprot | P63279, |
| Species.1 | Mouse |
| Gene ID.1 | 100044900 |
| Uniprot.1 | P63280, |
| Species.2 | Rat |
| Gene ID.2 | 25573 |
| Uniprot.2 | P63281 |
| Cellular Localization | Nucleus . Cytoplasm . Cytoplasm, perinuclear region . Mainly nuclear (By similarity). In spermatocytes, localizes in synaptonemal complexes (PubMed:8610150). Recruited by BCL11A into the nuclear body (By similarity). . |
| Tissue Expression | Expressed in heart, skeletal muscle, pancreas, kidney, liver, lung, placenta and brain. Also expressed in testis and thymus. |
| Signaling_pathway | Human Diseases >> Cancer: overview >> MicroRNAs in cancer;Environmental Information Processing >> Signal transduction >> NF-kappa B signaling pathway;Genetic Information Processing >> Translation >> Nucleocytoplasmic transport;Genetic Information Processing >> Folding, sorting and degradation >> Ubiquitin mediated proteolysis |
| Research Areas | >>Nucleocytoplasmic transport; / >>NF-kappa B signaling pathway; / >>Ubiquitin mediated proteolysis; / >>MicroRNAs in cancer |
| Function | Catalytic activity:ATP + SUMO + protein lysine = AMP + diphosphate + protein N-SUMOyllysine.,Function:Accepts the ubiquitin-like proteins SUMO1, SUMO2, SUMO3 and SUMO4 from the UBLE1A-UBLE1B E1 complex and catalyzes their covalent attachment to other proteins with the help of an E3 ligase such as RANBP2 or CBX4. Essential for nuclear architecture and chromosome segregation.,pathway:Protein modification; protein sumoylation.,similarity:Belongs to the ubiquitin-conjugating enzyme family.,subunit:Interacts with HIPK1, HIPK2 and PPM1J (By similarity). Forms a tight complex with RANGAP1 and RANBP2. Interacts with SIAH1 and PARP. Interacts with various transcription factors such as TCF3, TFAP2A, TFAP2B, TFAP2C, AR, ETS1 and SOX4. Interacts with human adenovirus E1A and human herpesvirus 6 IE2. Interacts with RWDD3; the interaction enhances the sumoylation of a number of proteins such as HIF1A and I-kappa-B.,tissue specificity:Expressed in heart, skeletal muscle, pancreas, kidney, liver, lung, placenta and brain. Also expressed in testis and thymus., |
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