U-Blot® ULA1 Rabbit mAb
Ucallm_WR6063-50
Price range: $268.00 through $328.00
Product details
Background：
The protein encoded by this gene binds to the beta-amyloid precursor protein. Beta-amyloid precursor protein is a cell surface protein with signal-transducing properties , and it is thought to play a role in the pathogenesis of Alzheimer's disease. In addition , the encoded protein can form a heterodimer with UBE1C and bind and activate NEDD8 , a ubiquitin-like protein. This protein is required for cell cycle progression through the S/M checkpoint. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq , Jul 2008] ,
Specifications
| Size | 50μL/100μL |
| Target | ULA1 |
| Reactivity | Human, Rat |
| Applications | WB, IHC, IF, IP, ELISA |
| MW(Calculated) | 60kD |
| MW(Observed) | 60kD |
| Host Species | Rabbit |
| Isotype | IgG,Kappa |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | IHC 1:200-1:1000; WB 1:2000-1:10000; IF 1:200-1:1000; ELISA 1:5000-1:20000; IP 1:50-1:200; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Purification | Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | RM1976R |
| Immunogen | The specific immunogen used to produce this antibody is proprietary information. |
| Specificity | Endogenous |
| Gene Name | NAE1 APPBP1 HPP1 |
| Protein Name | NEDD8-activating enzyme E1 regulatory subunit (Amyloid beta precursor protein-binding protein 1, 59 kDa) (APP-BP1) (Amyloid protein-binding protein 1) (Proto-oncogene protein 1) |
| Other Name | |
| Species | Human |
| Gene ID | 8883 |
| Uniprot | Q13564, |
| Species.1 | Mouse |
| Gene ID.1 | |
| Uniprot.1 | Q8VBW6, |
| Species.2 | Rat |
| Gene ID.2 | |
| Uniprot.2 | Q9Z1A5 |
| Cellular Localization | Cell membrane . Colocalizes with APP in lipid rafts. |
| Tissue Expression | Ubiquitous in fetal tissues. Expressed throughout the adult brain. |
| Signaling_pathway | Human Diseases >> Neurodegenerative disease >> Alzheimer disease |
| Research Areas | >>Alzheimer disease |
| Function | Caution:The sequence shown here is derived from an Ensembl automatic analysis pipeline and should be considered as preliminary data.,enzyme regulation:Binding of TP53BP2 to the regulatory subunit NAE1 decreases neddylation activity.,Function:Regulatory subunit of the dimeric UBA3-NAE1 E1 enzyme. E1 activates NEDD8 by first adenylating its C-terminal glycine residue with ATP, thereafter linking this residue to the side chain of the catalytic cysteine, yielding a NEDD8-UBA3 thioester and free AMP. E1 finally transfers NEDD8 to the catalytic cysteine of UBE2M. Necessary for cell cycle progression through the S-M checkpoint. Overexpression of NAE1 causes apoptosis through deregulation of NEDD8 conjugation.,miscellaneous:NAE1 and UBA3 correspond to the N-terminal and the C-terminal part of yeast UBA3. In yeast the two subunits form a single polypeptide chain.,pathway:Protein modification; protein neddylation.,similarity:Belongs to the ubiquitin-activating E1 family. ULA1 subfamily.,subcellular location:Colocalizes with APP in lipid rafts.,subunit:Heterodimer of UBA3 and NAE1. The complex binds NEDD8 and UBE2M. Binds APP and TP53BP2.,tissue specificity:Ubiquitous in fetal tissues. Expressed throughout the adult brain., |
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