U-Blot® V ATPase B1 Rabbit mAb
Ucallm_WR6032-50
Price range: $268.00 through $328.00
Product details
Background：
This gene encodes a component of vacuolar ATPase (V-ATPase) , a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting , zymogen activation , receptor-mediated endocytosis , and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits , two G subunits plus the C , D , E , F , and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a , c , c' , c'' , and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This encoded protein is one of two V1 domain B subunit isoforms and is found i
Specifications
| Size | 50μL/100μL |
| Target | V-ATPase B1 |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC, IF, ELISA |
| MW(Calculated) | 57kD |
| MW(Observed) | 55kD |
| Host Species | Rabbit |
| Isotype | IgG,Kappa |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | IHC 1:200-1:1000; WB 1:2000-1:10000; IF 1:200-1:1000; ELISA 1:5000-1:20000; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Purification | Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | RM2042R |
| Immunogen | The specific immunogen used to produce this antibody is proprietary information. |
| Specificity | Endogenous |
| Gene Name | ATP6V1B1 |
| Protein Name | V-type proton ATPase subunit B kidney isoform |
| Other Name | ATP6V1B1; / ATP6B1; / VATB; / VPP3; / V-type proton ATPase subunit B; / kidney isoform; / V-ATPase subunit B 1; / Endomembrane proton pump 58 kDa subunit; / Vacuolar proton pump subunit B 1 |
| Species | Human |
| Gene ID | 525 |
| Uniprot | P15313 |
| Species.1 | |
| Gene ID.1 | |
| Uniprot.1 | |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | Apical cell membrane . Basolateral cell membrane . |
| Tissue Expression | Kidney; localizes to early distal nephron, encompassing thick ascending limbs and distal convoluted tubules (at protein level) (PubMed:29993276, PubMed:16769747). Expressed in the cochlea and endolymphatic sac (PubMed:9916796). |
| Signaling_pathway | Cellular Processes >> Transport and catabolism >> Phagosome;Organismal Systems >> Nervous system >> Synaptic vesicle cycle;Human Diseases >> Immune disease >> Rheumatoid arthritis;Environmental Information Processing >> Signal transduction >> mTOR signaling pathway |
| Research Areas | >>Oxidative phosphorylation; / >>Metabolic pathways; / >>Phagosome; / >>mTOR signaling pathway; / >>Synaptic vesicle cycle; / >>Collecting duct acid secretion; / >>Vibrio cholerae infection; / >>Epithelial cell signaling in Helicobacter pylori infection; / >>Human papillomavirus infection; / >>Rheumatoid arthritis |
| Function | Disease:Defects in ATP6V1B1 are the cause of distal renal tubular acidosis with deafness (dRTA) [MIM:267300]. Inheritance is autosomal recessive. Patients with recessive dRTA are severely affected, presenting with either acute illness or growth failure at a young age, and bilateral sensorineural deafness. Other features include low serum K(+) due to renal potassium wasting, and elevated urinary calcium. If untreated, this acidosis may result in dissolution of bone, leading to osteomalacia and rickets. Renal deposition of calcium salts (nephrocalcinosis) and renal stone formation commonly occur.,Domain:The PDZ-binding motif mediates interactions with SLC9A3R1 and SCL4A7.,Function:Non-catalytic subunit of the peripheral V1 complex of vacuolar ATPase. V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells.,similarity:Belongs to the ATPase alpha/beta chains family.,subcellular location:Endomembrane.,subunit:V-ATPase is an heteromultimeric enzyme composed of a peripheral catalytic V1 complex (main components: subunits A, B, C, D, E, and F) attached to an integral membrane V0 proton pore complex (main component: the proteolipid protein). Forms a complex with SLC9A3R1 and SCL4A7.,tissue specificity:Expressed in the cochlea and endolymphatic sac., |
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