Name: U-Blot® VATA Rabbit mAb
SKU: WR6010
Price: 268 USD
Availability: InStock

Product details

Background：
This gene encodes a component of vacuolar ATPase (V-ATPase) , a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting , zymogen activation , receptor-mediated endocytosis , and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits , two G subunits plus the C , D , E , F , and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a , c , c' , c" , and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This encoded protein is one of two V1 domain A subunit isoforms and is found in all

Specifications

Target	VATA
Reactivity	Human, Mouse, Rat
Applications	WB, IHC, IF, IP, ELISA
MW(Calculated)	68kD
MW(Observed)	68kD
Host Species	Rabbit
Isotype	IgG,Kappa
Conjugate/Modification	Unmodified
Modification
Recommended Dilution Ratio	IHC 1:200-1:1000; WB 1:2000-1:10000; IF 1:200-1:1000; ELISA 1:5000-1:20000; IP 1:50-1:200; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#YS0004)
Formulation	PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
Source
Purification	Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography.
Purity
Storage	-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration
Clonality	Monoclonal
Clone Number	PT2004R
Immunogen	The specific immunogen used to produce this antibody is proprietary information.
Sequence
Specificity	Endogenous
Gene Name	ATP6V1A ATP6A1 ATP6V1A1 VPP2
Protein Name	V-type proton ATPase catalytic subunit A (V-ATPase subunit A) (V-ATPase 69 kDa subunit) (Vacuolar ATPase isoform VA68) (Vacuolar proton pump subunit alpha)
Other Name
Species	Human
Gene ID-1	523
Uniprot	P38606,
Species.1	Mouse
Gene ID-2
Uniprot.1	P50516
Species.2
Gene ID-3
Uniprot.2
Organism-4
Gene ID-4
SwissProt-4
Background	This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c' , c" , and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This encoded protein is one of two V1 domain A subunit isoforms and is found in all
Cellular Localization	Cytoplasm . Cytoplasm, cytosol . Cytoplasmic vesicle, secretory vesicle . Cytoplasmic vesicle, clathrin-coated vesicle membrane; Peripheral membrane protein . Lysosome . Co-localizes with WFS1 in the secretory granules in neuroblastoma cell lines. .
Tissue Expression	High expression in the skin.
Signaling_pathway	Cellular Processes >> Transport and catabolism >> Phagosome;Organismal Systems >> Nervous system >> Synaptic vesicle cycle;Human Diseases >> Immune disease >> Rheumatoid arthritis;Environmental Information Processing >> Signal transduction >> mTOR signaling pathway
Research Areas	>>Oxidative phosphorylation; / >>Metabolic pathways; / >>Phagosome; / >>mTOR signaling pathway; / >>Synaptic vesicle cycle; / >>Collecting duct acid secretion; / >>Vibrio cholerae infection; / >>Epithelial cell signaling in Helicobacter pylori infection; / >>Human papillomavirus infection; / >>Rheumatoid arthritis
Function	Catalytic activity:ATP + H(2)O + H(+)(In) = ADP + phosphate + H(+)(Out).,Function:Catalytic subunit of the peripheral V1 complex of vacuolar ATPase. V-ATPase vacuolar ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells.,similarity:Belongs to the ATPase alpha/beta chains family.,subunit:V-ATPase is an heteromultimeric enzyme composed of a peripheral catalytic V1 complex (main components: subunits A, B, C, D, E, and F) attached to an integral membrane V0 proton pore complex (main component: the proteolipid protein).,tissue specificity:Present in all tissues analyzed.,
RRID