U-Blot® Villin Rabbit mAb
Ucallm_WR5217-50
Price range: $268.00 through $328.00
Product details
Background:This gene encodes a member of a family of calcium-regulated actin-binding proteins. This protein represents a dominant part of the brush border cytoskeleton which functions in the capping, severing, and bundling of actin filaments. Two mRNAs of 2.7 kb and 3.5 kb have been observed; they result from utilization of alternate poly-adenylation signals present in the terminal exon. [provided by RefSeq, Jul 2008],
Specifications
| Size | 50μL/100μL |
| Target | Villin |
| Reactivity | Human |
| Applications | WB, IHC, IF, ELISA |
| MW(Calculated) | 93kD |
| MW(Observed) | 93kD |
| Host Species | Rabbit |
| Isotype | IgG,Kappa |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | IHC 1:200-1:1000; WB 1:2000-1:10000; IF 1:200-1:1000; ELISA 1:5000-1:20000; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Purification | Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | RM1451R |
| Immunogen | The specific immunogen used to produce this antibody is proprietary information. |
| Specificity | Endogenous |
| Gene Name | VIL1 VIL |
| Protein Name | Villin-1 |
| Other Name | villin 1 |
| Species | Human |
| Gene ID | 7429 |
| Uniprot | P09327, |
| Species.1 | Mouse |
| Gene ID.1 | 22349 |
| Uniprot.1 | Q62468 |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | Cytoplasm, cytoskeleton. Cell projection, lamellipodium. Cell projection, ruffle. Cell projection, microvillus. Cell projection, filopodium tip . Cell projection, filopodium . Relocalized in the tip of cellular protrusions and filipodial extensions upon infection with S.flexneri in primary intestinal epithelial cells (IEC) and in the tail-like structures forming the actin comets of S.flexneri. Redistributed to the leading edge of hepatocyte growth factor (HGF)-induced lamellipodia (By similarity). Rapidly redistributed to ruffles and lamellipodia structures in response to autotaxin, lysophosphatidic acid (LPA) and epidermal growth factor (EGF) treatment. . |
| Tissue Expression | Specifically expressed in epithelial cells. Major component of microvilli of intestinal epithelial cells and kidney proximal tubule cells. Expressed in canalicular microvilli of hepatocytes (at protein level). |
| Signaling_pathway | |
| Research Areas | |
| Function | Domain:Consists of a large core fragment, the N-terminal portion, and a small headpiece, the C-terminal portion. The headpiece binds F-actin strongly in both the presence and absence of calcium.,Function:Ca(2+)-regulated actin-binding protein.,similarity:Belongs to the villin/gelsolin family.,similarity:Contains 1 HP (headpiece) domain.,similarity:Contains 6 gelsolin-like repeats.,subunit:Monomer.,tissue specificity:Major component of microvilli of intestinal epithelial cells and kidney proximal tubule cells., |
Please login to post questions
Related products
