Price range: $268.00 through $328.00
Background:
Posttranslational modification of proteins by the addition of the small protein SUMO (see SUMO1; MIM 601912), or sumoylation, regulates protein structure and intracellular localization. SAE1 (MIM 613294) and UBA2 form a heterodimer that functions as a SUMO-activating enzyme for the sumoylation of proteins (Okuma et al., 1999 [PubMed 9920803]).[supplied by OMIM, Mar 2010],
| Size | 50μL/100μL |
| Target | UBA2 |
| Reactivity | Human, Mouse |
| Applications | WB, IHC, IF, ELISA |
| MW(Calculated) | |
| MW(Observed) | 71kD |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:500-1:2000; IHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:10000; Not yet tested in other applications. / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human Uba2. AA range:591-640 |
| Specificity | UBA2 Polyclonal Antibody detects endogenous levels of UBA2 protein. |
| Gene Name | UBA2 |
| Protein Name | SUMO-activating enzyme subunit 2 |
| Other Name | UBA2; / SAE2; / UBLE1B; / HRIHFB2115; / SUMO-activating enzyme subunit 2; / Anthracycline-associated resistance ARX; / Ubiquitin-like 1-activating enzyme E1B |
| Species | Human |
| Gene ID | 10054 |
| Uniprot | Q9UBT2, |
| Species.1 | Mouse |
| Gene ID.1 | |
| Uniprot.1 | Q9Z1F9 |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | Cytoplasm. Nucleus. Shuttles between the cytoplasm and the nucleus, sumoylation is required either for nuclear translocation or nuclear retention. |
| Tissue Expression | Brain,Cervix carcinoma,Fetal brain,Placenta,Skin,Testis, |
| Signaling_pathway | Genetic Information Processing >> Folding, sorting and degradation >> Ubiquitin mediated proteolysis |
| Research Areas | >>Ubiquitin mediated proteolysis |
| Function | Function:The dimeric enzyme acts as a E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins and formation of a thioester with a conserved cysteine residue on SAE2.,pathway:Protein modification; protein sumoylation.,similarity:Belongs to the ubiquitin-activating E1 family.,subunit:Heterodimer of SAE1 and SAE2. The complex binds SUMO proteins via SAE2., |
