Price range: $268.00 through $328.00
Background:
This gene encodes a member of the E1-like ubiquitin-activating enzyme family. This protein activates ubiquitin-fold modifier 1, a ubiquitin-like post-translational modifier protein, via the formation of a high-energy thioester bond. Alternative splicing results in multiple transcript variants. A pseudogene of this gene has been identified on chromosome 1. [provided by RefSeq, Feb 2016],
| Size | 50μL/100μL |
| Target | Uba5 |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC, IF, ELISA |
| MW(Calculated) | |
| MW(Observed) | 45kD |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:5000; IF 1:50-200 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human UBA5. AA range:281-330 |
| Specificity | Uba5 Polyclonal Antibody detects endogenous levels of Uba5 protein. |
| Gene Name | UBA5 |
| Protein Name | Ubiquitin-like modifier-activating enzyme 5 |
| Other Name | UBA5; / UBE1DC1; / Ubiquitin-like modifier-activating enzyme 5; / Ubiquitin-activating enzyme 5; / ThiFP1; / UFM1-activating enzyme; / Ubiquitin-activating enzyme E1 domain-containing protein 1 |
| Species | Human |
| Gene ID | 79876 |
| Uniprot | Q9GZZ9, |
| Species.1 | Mouse |
| Gene ID.1 | 66663 |
| Uniprot.1 | Q8VE47, |
| Species.2 | Rat |
| Gene ID.2 | 300968 |
| Uniprot.2 | Q5M7A4 |
| Cellular Localization | Cytoplasm . Nucleus . Endoplasmic reticulum membrane . Golgi apparatus . Localizes mainly in the cytoplasm, while it localizes to the nucleus in presence of SUMO2 (PubMed:18442052). Interaction with GABARAPL2 promotes localization to the endoplasmic reticulum membrane (PubMed:30990354). . |
| Tissue Expression | Widely expressed. |
| Signaling_pathway | |
| Research Areas | |
| Function | Function:E1-like enzyme which activates UFM1.,similarity:Belongs to the ubiquitin-activating E1 family. UBA5 subfamily., |
