Name: U-Blot® UBA52 Rabbit mAb
SKU: WR4274
Price: 268 USD
Availability: InStock

U-Blot® UBA52 Rabbit mAb

U-251 MG whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with primary antibody(1:1000). The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody.

WR4274 U-251 MG whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with primary antibody(1:1000). The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody.

Price range: $268.00 through $328.00

Size

100μl

50μl

Clear

SKU: WR4274-50

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Product details

Background:Ubiquitin is a highly conserved nuclear and cytoplasmic protein that has a major role in targeting cellular proteins for degradation by the 26S proteosome. It is also involved in the maintenance of chromatin structure, the regulation of gene expression, and the stress response. Ubiquitin is synthesized as a precursor protein consisting of either polyubiquitin chains or a single ubiquitin moiety fused to an unrelated protein. This gene encodes a fusion protein consisting of ubiquitin at the N terminus and ribosomal protein L40 at the C terminus, a C-terminal extension protein (CEP). Multiple processed pseudogenes derived from this gene are present in the genome. [provided by RefSeq, Jul 2008],

Specifications

Size	50μL/100μL
Target	UBA52
Reactivity	Human, Mouse, Rat
Applications	WB, IHC, IF, IP, ELISA
MW(Calculated)	15kD
MW(Observed)	10kD
Host Species	Rabbit
Isotype	IgG,Kappa
Conjugate/Modification	Unmodified
Recommended Dilution Ratio	IHC 1:200-1:1000; WB 1:2000-1:10000; IF 1:200-1:1000; ELISA 1:5000-1:20000; IP 1:50-1:200; / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004)
Formulation	PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA
Purification	Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography.
Storage	-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration
Clonality	Monoclonal
Clone Number	RM0081R
Immunogen	The specific immunogen used to produce this antibody is proprietary information.
Specificity	Endogenous
Gene Name	UBA52
Protein Name	Ubiquitin-60S ribosomal protein L40
Other Name	UBA52; / UBCEP2; / Ubiquitin-60S ribosomal protein L40; / CEP52; / Ubiquitin A-52 residue ribosomal protein fusion product 1
Species	Human
Gene ID	5816
Uniprot	P62987,
Species.1	Mouse
Gene ID.1	22186
Uniprot.1	P62984,
Species.2	Rat
Gene ID.2
Uniprot.2	P02625
Cellular Localization	[Ubiquitin]: Cytoplasm . Nucleus .; [60S ribosomal protein L40]: Cytoplasm .
Tissue Expression	Cerebellum,
Signaling_pathway
Research Areas
Function	Function:Protein modifier which can be covalently attached to target lysines either as a monomer or as a lysine-linked polymer. Attachment to proteins as a Lys-48-linked polymer usually leads to their degradation by proteasome. Attachment to proteins as a monomer or as an alternatively linked polymer does not lead to proteasomal degradation and may be required for numerous functions, including maintenance of chromatin structure, regulation of gene expression, stress response, ribosome biogenesis and DNA repair.,miscellaneous:This ribosomal protein is synthesized as a C-terminal extension protein (CEP) of ubiquitin.,miscellaneous:Ubiquitin is synthesized as a polyubiquitin precursor with exact head to tail repeats, the number of repeats differ between species and strains. In some species there is a final amino-acid after the last repeat, here in human a Val. Some ubiquitin genes contain a single copy of ubiquitin fused to a ribosomal protein (either L40 or S27a).,PTM:Several types of polymeric chains can be formed, depending on the lysine used for the assembly.,similarity:Belongs to the ribosomal protein L40e family.,similarity:Belongs to the ribosomal protein S27Ae family.,similarity:Belongs to the ubiquitin family.,