U-Blot™ Phosphate Buffered Saline with Tween 20 (PBS-T), pH 7.4
Product details
PBST (Phosphate Buffered Saline with Tween-20) is a phosphate-buffered saline containing Tween-20, widely used in biological experiments. It provides a stable ionic environment and pH buffering capacity. PBST is primarily used in immunohistochemistry, in situ hybridization, ELISA, Western blotting, and washing immunoblot membranes.
The inclusion of Tween-20, a non-ionic detergent, reduces non-specific binding and protein-protein interactions during washing steps, improving clarity and specificity in experimental results.
Storage and Stability:
Store in a dry, sealed container at room temperature (2–30°C) away from light. Shelf life: up to 3 years.
Specifications
| Specifications | PBST pH 7.4 |
| Grade | Biotechnology / Analytical grade |
| Format | Tablets, Exactly premixed Instant Granules 0.137 M NaCl |
| Composition | 0.0027 M KCl 0.010 M PO43- 0.05% Tween 20 |
| Volume | 100 ml/Tablet , 1L/Pouch, 25Kg/Pack |
| pH(@25°C) | 7.40 ± 0.20 |
| Shelf life | Three years after the production date |
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