Product details
Nucleoplasm, also referred to as karyoplasm/nucleus sap, is consisted of chromosomes, nucleolus and nuclear matrix. Nucleoplasm plays a central role in genetic information flow and regulation of gene expression. Nuclear protein isolation has been traditionally accomplished by extraction protocol using detergent-containing buffers, which is effective in extracting total nuclear proteins but unable to separate membrane-bound proteins and water-soluble matrix proteins. The detergent-free nuclear matrix protein extraction kit is designed to separate cellular protein into three fractions: cytosolic, nuclear matrix and water-insoluble fraction (mainly envelope and nucleic acid-associated proteins) using a patented spin column-based technology. The buffers used are detergent and EDTA- free. The whole protocol can be completed in less than 1h. The proteins extracted are in their native state and can be used in many downstream applications such as gel retardation, transcription factor analysis, apoptosis, and protein trafficking studies.
