Product details
U-Blot™ detergent-free total protein extraction kit is composed of optimized cell lysis buffer and protein extraction filter cartridges with 2.0 ml collection tubes. The kit is designed to rapidly extract total proteins from cultured cells (insect /mammalian/other cultured cells) and animal tissues (invertebrate and vertebrate). The protein extraction buffers do not contain any detergent and EDTA. Due to the use of the protein extraction filter cartridges, the extraction volume can be as small as 20 µl and as large as 500 µl. This unique feature is very useful in situations where available starting material is a limiting factor. Detergent-free total proteins can be extracted from cultured cells/tissues in less than 5 min with high yield (1-5 mg/ml).
Application
U-Blot™ detergent-free total protein extraction kit is designed to rapidly extract total proteins from invertebrate and vertebrate cultured cells/tussues for applications such as proteomics (LC/MS), IP, ELISA, 2D-gel analysis, isoelectric focusing, SDS-PAGE, immunoblottings, and other applications. This kit provides a very rapid method for preparation of high concentration of whole cell extract. Because of high yield of protein extract and low salt concentration, the protein extract can be used directly for downstream applications without desalting and protein concentration.
