Product details
U-Blot™ mitochondria isolation kit is composed of optimized buffers and protein extraction filter cartridges with 2.0 ml collection tubes. The kit is designed to rapidly isolate intact native mitochondria from cultured mammalian cells or tissues. Due to the use of protein extraction filter cartridges, the protein isolation procedure is simple, easy and user friendly with high yield. Unlike other commercial mitochondrial preparation kits, this kit offers wide range of starting cells (5-40 millions/sample). The buffers are detergent and EDTA free. A Dounce homogenizer or a tissue blender is not needed. The procedure can be completed in about 30 min.
Applications
The kit is designed to rapidly isolate intact mitochondria from cultured cells or tissues for applications such as SDS-PAGE, immunoblottings, ELISA, IP, 2-D gels, membrane potential assays, enzyme activity assays and other applications.
