Product details
Isolation of microsomal membranes from plant tissues is a common laboratory procedure. Microsomal fraction of plant cell lysate is the focus of interest in many plant research projects. Microsomal fraction is believed to be enriched for plasma membranes, endoplasmic reticulum, Golgi apparatus, vacuolar membranes and other components of membrane system. Traditional method for microsomal fraction isolation involves so called differential pelleting protocol where a series of centrifugation steps are required to obtain various membrane fractions. Traditional protocol for microsomal fraction isolation requires large amount of starting material and employs tedious ultracentrifugation steps. MM-018 offers a simple, rapid and user friendly approach for microsomal membrane extraction using small amount of starting material (200 mg). Water soluble cytosolic proteins are removed during the procedures and water insoluble microsomal fraction, especially plasma membrane fraction, is extracted with optimized buffers in a table-top microcentrifuge. The procedure is simple, rapid and no special instrument required. Native microsomal proteins can be isolated from plant tissue in about one hour without ultracentrifugation. The protein yield is in the range of 100-200 µg/sample.
Applications
Microsomal membranes extracted with this kit can be used for many downstream applications such as SDS-PAGE analysis, Western blotting, IP. ELISA, enzyme activity assays, proteomics and membrane trafficking analysis.
