Product details
Bone tissues are commonly used for research. There are two types of bone tissues: compact and spongy. Cells in bone tissues are tightly packed. Because of their unique structures it is very difficult to efficiently extract total protein from them. The traditional solution-based protein extraction method such as RIPA is inefficient and the protein yield is very low. The profile of extracted protein is also incomplete with solution-based methods. This kit provides a highly efficient method for extracting proteins from human or animal bone tissues by a combination of mechanical extraction and chemical lysis of cells in tissues. The kit features a simple and rapid single tube protocol and optimized buffers for bone tissues. The researchers have the option to choose either denaturing cell lysis buffer or native cell lysis buffer, which are specifically tailored for bone tissue. The whole procedure takes less than 10 min to complete and the protein yield is in the range of 0.5-2 mg/ml depending upon type of bones. The materials provided are sufficient for 50 extractions.
Applications
Proteins extracted with this kit can be used for many downstream applications such as SDS-PAGE analysis, Western blotting, IP. ELISA, enzyme activity assays and proteomic analysis. The buffers are compatible with IMAC resins for his-tagged protein purification. The salts and detergents in the extracted protein sample should be removed prior to mass spectrometry analysis.
