Product details
The U-Blot™ total protein extraction kit for plant tissues comprises an optimized protein extraction buffer and protein extraction filter cartridges accompanied by 2.0 ml collection tubes. Its aims to efficiently extract denatured or native proteins from various plant tissues, including leaves, seeds, soft stems, and roots. It is worth noting that the protein profiles obtained through denaturing and native cell lysis buffers differ. Therefore, depending on the specific application, one buffer may outperform the other. This kit offers both denaturing and native cell lysis buffers, allowing users to evaluate and select the most suitable option for their particular needs. By utilizing the protein extraction filter cartridges, it becomes possible to extract total plant soluble proteins from 50-200 mg of plant tissue within a short span of 5-8 minutes, while achieving a high protein yield ranging from 2-8 mg/ml.
Application
The U-Blot™ total protein extraction kit for plant tissues is specifically designed to swiftly extract total proteins from both fresh and frozen plant tissues. This extraction method is particularly useful for applications such as SDS- PAGE, immunoblotting, ELISA, IP, enzyme assays, and various other applications. The kit offers a highly efficient and expedited approach for obtaining soluble protein extracts from plant tissues. These extracted proteins can also serve as excellent starting materials for small-scale protein purification using column chromatography techniques.
