Product details
Skin tissue is consisted of epidermis, dermis and subcutaneous fat. Because of its unique structure, skin tissue is notoriously difficult to homogenize. It is also very difficult to lyse the cells in the tissue for total protein extraction. The traditional solution-based protein extraction method such as RIPA is inefficient and the yield is very low. The profile of extracted protein is also incomplete with solution-based methods. This kit provides a highly efficient method for total protein extraction from human or animal skin tissues by a combination of mechanical extraction and chemical lysis of skin tissues. The kit features a simple and rapid single tube protocol and optimized buffers for skin tissues. The researchers have the option to choose either denaturing cell lysis buffer or native cell lysis buffer, which are specifically tailored for skin tissue. The whole procedure takes less than 10 min to complete and the protein yield is in the range of 1-5 mg/ml. The materials provided are sufficient for 50 extractions.
Applications
Proteins extracted with this kit can be used for many downstream applications such as SDS-PAGE analysis, Western blotting, IP. ELISA, enzyme activity assays and proteomic analysis. The buffers are compatible with IMAC resins for his-tagged protein purification. The salts and detergents in the extracted protein sample should be removed prior to mass spectrometry analysis.
