Product details
A tendon/ligament is a fibrous connective tissue attaches muscles to bone. Histologically tendons are consisted of dense regular collagen fibers that are very tough and notoriously difficult to homogenize. It is also very difficult to lyse the tendon tissues for total protein extraction. The traditional solution-based protein extraction method such as RIPA is inefficient and protein yield is very low. The profile of extracted protein is also biased (incomplete protein profile) with solution-based methods. This kit provides a highly efficient method for total protein extraction from human or animal tendons by a combination of mechanical extraction and chemical lysis. The cell lysis buffers used are much stronger than RIPA buffer. The kit features a simple and rapid single tube protocol and optimized buffers for tendons. The researchers have the option to choose either denaturing cell lysis buffer or native cell lysis buffer, which are specifically tailored for tendons. The whole procedure takes less than 10 min to complete and the protein yield is in the range of 1-3 mg/ml. The materials provided are sufficient for 50 extractions.
Applications
Proteins extracted with this kit can be used for many downstream applications such as SDS-PAGE analysis, Western blotting, IP. ELISA, enzyme activity assays and proteomic analysis. The buffers are compatible with IMAC resins for his-tagged protein purification. The salts and detergents in the extracted protein sample should be removed prior to mass spectrometry analysis.
