U-Blot® ULK1 (Phospho Ser757) Rabbit mAb
WR4517 NIH-3T3 cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with primary antibody 1:1000. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody.
Price range: $288.00 through $368.00
Product details
Background:catalytic activity:ATP + a protein = ADP + a phosphoprotein.,function:Involved in axon growth. Plays an essential role in neurite extension of cerebellar granule cells.,similarity:Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. APG1/unc-51/ULK1 subfamily.,similarity:Contains 1 protein kinase domain.,subunit:Interacts with GABARAP and GABARAPL2.,tissue specificity:Ubiquitously expressed. Detected in the following adult tissues: skeletal muscle, heart, pancreas, brain, placenta, liver, kidney, and lung.,
Specifications
| Size | 50μL/100μL |
| Target | ULK1 Phospho Ser757 |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IF, IP, ELISA |
| MW(Calculated) | 113kD |
| MW(Observed) | 150kD |
| Host Species | Rabbit |
| Isotype | IgG,Kappa |
| Conjugate/Modification | phosphate |
| Recommended Dilution Ratio | WB 1:2000-1:10000; IF 1:200-1:1000; ELISA 1:5000-1:20000; IP 1:50-1:200; |
| Formulation | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Purification | Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein A affinity chromatography. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | RM0739R |
| Immunogen | The specific immunogen used to produce this antibody is proprietary information. |
| Specificity | The antibody detects endogenous ULK1(Phospho AE13) protein. |
| Gene Name | ULK1 |
| Protein Name | Serine/threonine-protein kinase ULK1 |
| Other Name | ULK1; / KIAA0722; / Serine/threonine-protein kinase ULK1; / Autophagy-related protein 1 homolog; / ATG1; / hATG1; / Unc-51-like kinase 1 |
| Species | Human |
| Gene ID | 8408 |
| Uniprot | O75385, |
| Species.1 | Mouse |
| Gene ID.1 | 22241 |
| Uniprot.1 | O70405 |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | Cytoplasm, cytosol . Preautophagosomal structure . Under starvation conditions, is localized to puncate structures primarily representing the isolation membrane that sequesters a portion of the cytoplasm resulting in the formation of an autophagosome. . |
| Tissue Expression | Ubiquitously expressed. Detected in the following adult tissues: skeletal muscle, heart, pancreas, brain, placenta, liver, kidney, and lung. |
| Signaling_pathway | Cellular Processes >> Transport and catabolism >> Autophagy - animal;Cellular Processes >> Transport and catabolism >> Mitophagy - animal;Organismal Systems >> Aging >> Longevity regulating pathway;Human Diseases >> Neurodegenerative disease >> Alzheimer disease;Human Diseases >> Neurodegenerative disease >> Amyotrophic lateral sclerosis;Human Diseases >> Neurodegenerative disease >> Huntington disease;Human Diseases >> Neurodegenerative disease >> Spinocerebellar ataxia;Human Diseases >> Neurodegenerative disease >> Pathways of neurodegeneration - multiple diseases;Environmental Information Processing >> Signal transduction >> AMPK signaling pathway;Environmental Information Processing >> Signal transduction >> mTOR signaling pathway |
| Research Areas | >>Mitophagy - animal; / >>Autophagy - animal; / >>mTOR signaling pathway; / >>AMPK signaling pathway; / >>Longevity regulating pathway; / >>Alzheimer disease; / >>Amyotrophic lateral sclerosis; / >>Huntington disease; / >>Spinocerebellar ataxia; / >>Pathways of neurodegeneration - multiple diseases |
| Function | Catalytic activity:ATP + a protein = ADP + a phosphoprotein.,Function:Involved in axon growth. Plays an essential role in neurite extension of cerebellar granule cells.,similarity:Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. APG1/unc-51/ULK1 subfamily.,similarity:Contains 1 protein kinase domain.,subunit:Interacts with GABARAP and GABARAPL2.,tissue specificity:Ubiquitously expressed. Detected in the following adult tissues: skeletal muscle, heart, pancreas, brain, placenta, liver, kidney, and lung., |
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