Price range: $268.00 through $328.00
Background:
Vimentin is the most common member of intermediate filament protein family. It is a main component of cytoskeleton structure. Its main function is to maintain cell integrity and skeleton stability. It is expressed in mesenchymal cells and mesoderm derived cells. Vimentin is a relatively specific marker of mesenchymal cells and their derived tumors.
| Size | 50μL/100μL |
| Target | Vimentin |
| Reactivity | Human, Mouse, Rat |
| Applications | IHC, WB, IF, ELISA |
| MW(Calculated) | 53kD |
| MW(Observed) | 54kD |
| Host Species | Mouse |
| Isotype | IgG2b,Kappa |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | IHC 1:200-1000; WB 1:500-2000; IF 1:100-500; ELISA 1:1000-5000 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
| Purification | Recombinant Antibody expressed in animal component-free (ACF) media, purified via Protein G affinity chromatography. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | |
| Clonality | Monoclonal |
| Clone Number | RMM019 |
| Immunogen | Synthesized peptide derived from human protein. AA range:400-466 |
| Specificity | The antibody can specifically recognize human Vimentin protein. In western blotting of HEK293 cell lysate, the antibody can label a 54 kDa band corresponding to Vimentin. |
| Gene Name | VIM |
| Protein Name | |
| Other Name | CTRCT30; / Epididymis luminal protein 113; / FLJ36605; / HEL113; / VIM; / VIME_HUMAN; / Vimentin |
| Species | Human |
| Gene ID | 7431 |
| Uniprot | P08670, |
| Species.1 | Mouse |
| Gene ID.1 | 22352 |
| Uniprot.1 | P20152, |
| Species.2 | Rat |
| Gene ID.2 | 81818 |
| Uniprot.2 | P31000 |
| Cellular Localization | Cytoplasmic |
| Tissue Expression | Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone-independent mammary carcinoma cell lines. |
| Signaling_pathway | Human Diseases >> Cancer: overview >> MicroRNAs in cancer |
| Research Areas | >>Epstein-Barr virus infection; / >>MicroRNAs in cancer |
| Function | Function:Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells.,online information:Vimentin entry,PTM:One of the most prominent phosphoproteins in various cells of mesenchymal origin. Phosphorylation is enhanced during cell division, at which time vimentin filaments are significantly reorganized.,sequence Caution:Intron retention.,similarity:Belongs to the intermediate filament family.,subunit:Homopolymer. Interacts with HCV core protein. Interacts with LGSN and SYNM.,tissue specificity:Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone-independent mammary carcinoma cell lines., |
