Price range: $268.00 through $328.00
Background:
The protein encoded by this gene is a member of the pexin family. It is found in serum and tissues and promotes cell adhesion and spreading, inhibits the membrane-damaging effect of the terminal cytolytic complement pathway, and binds to several serpin serine protease inhibitors. It is a secreted protein and exists in either a single chain form or a clipped, two chain form held together by a disulfide bond. [provided by RefSeq, Jul 2008],
| Size | 50μL/100μL |
| Target | Vitronectin |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, ELISA |
| MW(Calculated) | |
| MW(Observed) | 55kD |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:500-1:2000; ELISA 1:10000; Not yet tested in other applications. |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human Vitronectin. AA range:209-258 |
| Specificity | Vitronectin Polyclonal Antibody detects endogenous levels of Vitronectin protein. |
| Gene Name | VTN |
| Protein Name | Vitronectin |
| Other Name | VTN; / Vitronectin; / VN; / S-protein; / Serum-spreading factor; / V75 |
| Species | Human |
| Gene ID | 7448 |
| Uniprot | P04004, |
| Species.1 | Mouse |
| Gene ID.1 | 22370 |
| Uniprot.1 | P29788 |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | Secreted, extracellular space .; Parasitophorous vacuole . (Microbial infection) In P.falciparum-infected red blood cells, VTN internalization is detected at the early trophozoite stage (PubMed:29567995). Colocalizes with SERA5 at the schizont stage and with SERA5 P47 at the merozoite surface (PubMed:29567995). . |
| Tissue Expression | Expressed in the retina pigment epithelium (at protein level) (PubMed:25136834). Expressed in plasma (at protein level) (PubMed:2448300). Expressed in serum (at protein level) (PubMed:29567995). |
| Signaling_pathway | Cellular Processes >> Cellular community - eukaryotes >> Focal adhesion;Organismal Systems >> Immune system >> Complement and coagulation cascades;Environmental Information Processing >> Signal transduction >> PI3K-Akt signaling pathway;Environmental Information Processing >> Signaling molecules and interaction >> ECM-receptor interaction |
| Research Areas | >>PI3K-Akt signaling pathway; / >>Focal adhesion; / >>ECM-receptor interaction; / >>Complement and coagulation cascades; / >>Human papillomavirus infection; / >>Proteoglycans in cancer |
| Function | Domain:The SMB domain mediates interaction with SERPINE1/PAI1. The heparin-binding domain mediates interaction with insulin.,Function:Somatomedin-B is a growth hormone-dependent serum factor with protease-inhibiting activity.,Function:Vitronectin is a cell adhesion and spreading factor found in serum and tissues. Vitronectin interact with glycosaminoglycans and proteoglycans. Is recognized by certain members of the integrin family and serves as a cell-to-substrate adhesion molecule. Inhibitor of the membrane-damaging effect of the terminal cytolytic complement pathway.,PTM:It has been suggested that the active SMB domain may be permitted considerable disulfide bond heterogeneity or variability, thus two alternate disulfide patterns based on 3D structures are described with 1 disulfide bond conserved in both.,PTM:N- and O-glycosylated.,PTM:Phosphorylation on Thr-69 and Thr-76 favors cell adhesion and spreading.,PTM:Sulfated on 2 tyrosine residues.,similarity:Contains 1 SMB (somatomedin-B) domain.,similarity:Contains 4 hemopexin-like domains.,subunit:Exists in two forms: a single chain 75 kDa form (V75) and a clipped form composed of two chains (65 kDa and 10 kDa) (V65+V10) which are held together by a disulfide bond. Interacts with SERPINE1/PAI1 and insulin.,tissue specificity:Plasma., |
