Price range: $268.00 through $328.00
Background:
This gene encodes for a protein that in yeast forms part of a trimeric vacuolar-protein-sorting complex that is required for retrograde transport of proteins from prevacuoles to the late Golgi compartment. As in yeast, mammalian Vps54 proteins contain a coiled-coil region and dileucine motifs. Alternative splicing results in multiple transcript variants encoding different isoforms [provided by RefSeq, Jul 2008],
| Size | 50μL/100μL |
| Target | VPS54 |
| Reactivity | Human, Mouse, Rat |
| Applications | IHC, IF, ELISA |
| MW(Calculated) | 111kD |
| MW(Observed) | |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | IHC 1:100-1:300; ELISA 1:40000; IF 1:50-200 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | Synthesized peptide derived from the Internal region of human VPS54. |
| Specificity | VPS54 Polyclonal Antibody detects endogenous levels of VPS54 protein. |
| Gene Name | VPS54 |
| Protein Name | Vacuolar protein sorting-associated protein 54 |
| Other Name | VPS54; / HCC8; / Vacuolar protein sorting-associated protein 54; / Hepatocellular carcinoma protein 8; / Tumor antigen HOM-HCC-8; / Tumor antigen SLP-8p |
| Species | Human |
| Gene ID | 51542 |
| Uniprot | Q9P1Q0, |
| Species.1 | Mouse |
| Gene ID.1 | |
| Uniprot.1 | Q5SPW0 |
| Species.2 | |
| Gene ID.2 | |
| Uniprot.2 | |
| Cellular Localization | Golgi apparatus, trans-Golgi network . Membrane . Associates with membranes in an EIPR1-independent manner. . |
| Tissue Expression | Hepatoma,Placenta,Skin,Testis, |
| Signaling_pathway | |
| Research Areas | |
| Function | Function:May be involved in retrograde transport from early and late endosomes to late Golgi.,similarity:Belongs to the VPS54 family.,subunit:Component of the Golgi-associated retrograde protein (GARP) complex, also called VFT (VPS fifty-three) complex, composed of VPS52, VPS53 and VPS54., |
