SKU: WM0691 Categories: ,

WDR77 mouse mAb

Price range: $268.00 through $328.00

SKU: WM0691-50
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Product details

Background:
The protein encoded by this gene is an androgen receptor coactivator that forms a complex with protein arginine methyltransferase 5, which modifies specific arginines to dimethylarginines in several spliceosomal Sm proteins. The encoded protein may be involved in the early stages of prostate cancer, with most of the protein being nuclear-localized in benign cells but cytoplasmic in cancer cells. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2015],

Specifications

Size50μL/100μL
TargetWDR77
ReactivityHuman, Mouse, Rat
ApplicationsWB, ICC
MW(Calculated)
MW(Observed)42kD
Host SpeciesMouse
Isotype
Conjugate/ModificationUnmodified
Recommended Dilution RatioWB 1:1000; ICC 1:100
FormulationLiquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
PurificationThe antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen.
Storage-15°C to -25°C/1 year(Do not lower than -25°C)
Concentration
ClonalityMonoclonal
Clone Number2D12
ImmunogenPurified recombinant human WDR77 protein fragments expressed in E.coli.
SpecificityThis antibody detects endogenous levels of WDR77 and does not cross-react with related proteins.
Gene Namemep50/wdr77
Protein Name
Other Name2610312E17Rik; / Androgen receptor cofactor p44; / C79984; / HKMT1069; / MEP 50; / MEP-50; / MEP50; / MEP50_HUMAN; / Methylosome protein 50; / MGC2722; / Nbla10071; / p44; / p44/Mep50; / RGD1310479; / RP11 552M11.3; / WD repeat containing protein 77; / WD repeat domain 77; / WD repeat-containing protein 77; / WDR77.
SpeciesHuman
Gene ID79084
UniprotQ9BQA1,
Species.1Mouse
Gene ID.1
Uniprot.1Q99J09
Species.2
Gene ID.2
Uniprot.2
Cellular LocalizationNucleus . Cytoplasm . Nuclear in Leydig cells and cytoplasmic in germ cells during fetal testicular development. In adult testis, predominantly nuclear. Subcellular location varies from nuclear to cytoplasmic in various tumors (PubMed:17437848). .
Tissue ExpressionHighly expressed in heart, skeletal muscle, spleen, testis, uterus, prostate and thymus. In testis, expressed in germ cells and Leydig cells, but not in peritubular myocytes, nor in Sertoli cells. Expressed in prostate cancers, in seminomas and in Leydig cell tumors.
Signaling_pathway
Research Areas
Functiondevelopmental stage:Expressed in Leydig cells during fetal testicular development, especially during the second semester. Germ cells expression is detected as early as 10 weeks of gestation.,Function:Component of the 20S PRMT5-containing methyltransferase complex, which modifies specific arginines to dimethylarginines in several spliceosomal Sm proteins. This modification targets Sm proteins to the survival of motor neurons (SMN) complex for assembly into small nuclear ribonucleoprotein core particles. Might play a role in transcription regulation.,similarity:Contains 5 WD repeats.,subcellular location:Nuclear in Leydig cells and cytoplasmic in germ cells during fetal testicular development. In adult testis, predominantly nuclear. Subcellular location varies from nuclear to cytoplasmic in various tumors.,subunit:Component of the methylosome, a 20S complex containing at least PRMT5, CLNS1A and WDR77. Directly interacts with PRMT5, as well as with several Sm proteins, including SNRPB and SNRPD2 and, more weakly, SNRPD3 and SNRPE. Interacts with SUZ12 and histone H2A/HIST2H2AC, but not with histones H2B, H3 nor H4. Interacts with CTDP1. Interacts with AR and NKX3-1.,tissue specificity:Highly expressed in heart, skeletal muscle, spleen, testis, uterus, prostate and thymus. In testis, expressed in germ cells and Leydig cells, but not in peritubular myocytes, nor in Sertoli cells. Expressed in prostate cancers, in seminomas and in Leydig cell tumors.,
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