XRCC1 Polyclonal Antibody
Ucallm_WP15029-50
Price range: $268.00 through $328.00
Product details
Background:
The protein encoded by this gene is involved in the efficient repair of DNA single-strand breaks formed by exposure to ionizing radiation and alkylating agents. This protein interacts with DNA ligase III, polymerase beta and poly (ADP-ribose) polymerase to participate in the base excision repair pathway. It may play a role in DNA processing during meiogenesis and recombination in germ cells. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity. [provided by RefSeq, Jul 2008],
Specifications
| Size | 50μL/100μL |
| Target | XRCC1 |
| Reactivity | Human, Mouse, Rat |
| Applications | WB, IHC, IF, ELISA |
| MW(Calculated) | |
| MW(Observed) | 70kD |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | Unmodified |
| Recommended Dilution Ratio | WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:10000; IF 1:50-200 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#WT1004) |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Storage | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | The antiserum was produced against synthesized peptide derived from human XRCC1. AA range:517-566 |
| Specificity | XRCC1 Polyclonal Antibody detects endogenous levels of XRCC1 protein. |
| Gene Name | XRCC1 |
| Protein Name | DNA repair protein XRCC1 |
| Other Name | XRCC1; / DNA repair protein XRCC1; / X-ray repair cross-complementing protein 1 |
| Species | Human |
| Gene ID | 7515 |
| Uniprot | P18887, |
| Species.1 | Mouse |
| Gene ID.1 | 22594 |
| Uniprot.1 | Q60596, |
| Species.2 | Rat |
| Gene ID.2 | 84495 |
| Uniprot.2 | Q9ESZ0 |
| Cellular Localization | Nucleus . Moves from the nucleoli to the global nuclear chromatin upon DNA damage. . |
| Tissue Expression | Expressed in fibroblasts, retinal pigmented epithelial cells and lymphoblastoid cells (at protein level). |
| Signaling_pathway | |
| Research Areas | >>Base excision repair |
| Function | Function:Corrects defective DNA strand-break repair and sister chromatid exchange following treatment with ionizing radiation and alkylating agents.,polymorphism:Carriers of the polymorphic Gln-399 allele may be at greater risk for tobacco- and age-related DNA damage.,PTM:Phosphorylation of Ser-371 causes dimer dissociation. Phosphorylation by CK2 promotes interaction with APTX and APLF.,PTM:Sumoylated.,similarity:Contains 2 BRCT domains.,subcellular location:Accumulates at sites of DNA damage.,subunit:Homodimer. Interacts with polynucleotide kinase (PNK), DNA polymerase-beta (POLB) and DNA ligase III (LIG3). Interacts with APTX and APLF., |
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