Product details
Background:
YTHD3 rabbit pAb
Ucallm_WP17908-50
Price range: $268.00 through $328.00
Specifications
| Target | YTHDF3 |
| Reactivity | Human, Mouse |
| Applications | IHC, WB |
| MW(Calculated) | 64kD |
| MW(Observed) | |
| Host Species | Rabbit |
| Isotype | IgG |
| Conjugate/Modification | Unmodified |
| Modification | |
| Recommended Dilution Ratio | WB 1:500-2000; IHC 1:50-200 / Note: For IHC, we suggest antigen retrieval with TE buffer pH 9.0 (Cat#YS0004) |
| Formulation | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
| Source | |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Purity | |
| Storage | -15°C to -25°C/1 year (Do not lower than -25°C) |
| Concentration | 1 mg/ml |
| Clonality | Polyclonal |
| Clone Number | |
| Immunogen | Synthesized peptide derived from human N-ternal YTHD3. AA range:25-105 |
| Sequence | |
| Specificity | This antibody detects endogenous levels of YTHD3 at Human, Mouse |
| Gene Name | YTHDF3 |
| Protein Name | YTH domain family protein 3 |
| Other Name | YTH domain family protein 3 |
| Species | Human |
| Gene ID-1 | 253943 |
| Uniprot | Q7Z739, |
| Species.1 | Mouse |
| Gene ID-2 | 229096 |
| Uniprot.1 | Q8BYK6 |
| Species.2 | |
| Gene ID-3 | |
| Uniprot.2 | |
| Organism-4 | |
| Gene ID-4 | |
| SwissProt-4 | |
| Background | |
| Cellular Localization | Cytoplasm , cytosol . Cytoplasm , P-body . Cytoplasm , Stress granule . |
| Tissue Expression | |
| Signaling_pathway | |
| Research Areas | |
| Function | Specifically recognizes and binds N6-methyladenosine (m6A) -containing RNAs , and regulates their stability . M6A is a modification present at internal sites of mRNAs and some non-coding RNAs and plays a role in mRNA stability and processing . Acts as a regulator of mRNA stability by promoting degradation of m6A-containing mRNAs via interaction with the CCR4-NOT complex or PAN3 . The YTHDF paralogs (YTHDF1 , YTHDF2 and YTHDF3) share m6A-containing mRNAs targets and act redundantly to mediate mRNA degradation and cellular differentiation . Acts as a negative regulator of type I interferon response by down-regulating interferon-stimulated genes (ISGs) expression: acts by binding to FOXO3 mRNAs (By similarity) . Binds to FOXO3 mRNAs independently of METTL3-mediated m6A modification (By similarity) . Can also act as a regulator of mRNA stability in cooperation with YTHDF2 by binding to m6A-containing mRNA and promoting their degradation . Recognizes and binds m6A-containing circular RNAs (circRNAs); circRNAs are generated through back-splicing of pre-mRNAs , a non-canonical splicing process promoted by dsRNA structures across circularizing exons . Promotes formation of phase-separated membraneless compartments , such as P-bodies or stress granules , by undergoing liquid-liquid phase separation upon binding to mRNAs containing multiple m6A-modified residues: polymethylated mRNAs act as a multivalent scaffold for the binding of YTHDF proteins , juxtaposing their disordered regions and thereby leading to phase separation . The resulting mRNA-YTHDF complexes then partition into different endogenous phase-separated membraneless compartments , such as P-bodies , stress granules or neuronal RNA granules . May also recognize and bind N1-methyladenosine (m1A) -containing mRNAs: inhibits trophoblast invasion by binding to m1A-methylated transcripts of IGF1R , promoting their degradation .; Has some antiviral activity against HIV-1 virus: incorporated into HIV-1 particles in a nucleocapsid-dependent manner and reduces viral infectivity in the next cycle of infection . May interfere with this early step of the viral life cycle by binding to N6-methyladenosine (m6A) modified sites on the HIV-1 RNA genome . |
| RRID |
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