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Immunohistochemical analysis of paraffin-embedded human brain tissue. 1, primary Antibody was diluted at 1:200(4～C,overnight). 2, EDTA pH 9.0 was used for antibody retrieval(>98～C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min).
Sku: WR4170
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U-Blot® p27 KIP 1 Rabbit mAb

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Sku: WR5120
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U-Blot® P2Y12 Rabbit mAb

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Sku: WR4898
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U-Blot® p300 Rabbit mAb

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Sku: WR5881
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U-Blot® p38 MAPK (Phospho Thr180/Tyr182) Rabbit mAb

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Sku: WR6102
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U-Blot® p38 MAPK (Phospho Thr180/Tyr182)Rabbit mAb

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Various whole cell lysates were separated by 4-20% SDS-PAGE, and the primary antibody was used at 4°C, over night with a 1:5000 dilution . The Dylight 800-conjugated Goat anti-Rabbit antibody(Cat:WS0446) was used to detect the antibody.
Sku: WR4250
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U-Blot® p38 Rabbit mAb

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Sku: WR4841
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U-Blot® p53 (Phospho Ser15) Rabbit mAb

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Sku: WR4991
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U-Blot® p53 (Phospho Ser392) Rabbit mAb

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Western Blot analysis using HEK293 was treated with Calyculin A(100nM) for 1 hour lysates,primary antibody was diluted at 1:1000, 4°over night, Dylight 800 secondary antibody(WS0446)was diluted at 1:10000, 37° 1hour.
Sku: WR4627
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U-Blot® p53 (Phospho Ser46) Rabbit mAb

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Sku: WR5017
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U-Blot® p53 (Phospho Ser6) Rabbit mAb

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Sku: WM1879
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U-Blot® p53 Mouse mAb

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HEK293 cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with primary antibody 1:1000. The HRP-conjugated Goat anti-Rabbit IgG(H + L) antibody was used to detect the antibody.
Sku: WR4502
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U-Blot® p53 Rabbit mAb

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