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- Experimental steps required
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  Preparation of polyacrylamide gel
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  Setup of transfer apparatus
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Tag: Western Blot Optimization

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Laboratory Technology

Why Is There No Signal in Western Blot? 5 Common Causes and Fixes

failed bands

Troubleshooting Western Blot signal issues? Learn the 5 common causes and fixes to optimize your results.

June 12, 2025

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Products
- Experimental steps required
  Sample preparation
  Preparation of polyacrylamide gel
  Loading and running the gel
  Setup of transfer apparatus
  Transferring from the gel to the membrane
  Verification and blocking
  Antibody incubation
  Membrane stripping (optional)
  Detection and data analysis
  
  Instruments
  Thermal control
  Electrophoresis cell
  Centrifuges
  Mixing
  Spectrometer
  Electrophoresis power supply series
  Imaging luminescence series
  Western blot transfer system
  
  Reagents
  Protein separation and extraction kit
  Lysis buffer
  Running buffer
  Transfer buffer
  Blocking solution
  Detection substrate
  SDS-PAGE gel
  Antibody
  
  Consumables
  Blotting membrane
Services
Applications
Resources
Partners

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