Background：Actin (pan) Rabbit mAb is a rabbit-derived monoclonal antibody that can recognize multiple actin isoforms, which are widely present in various cell types. As a commonly used internal reference in Western Blot (WB), it can calibrate sample loading amounts, with high specificity, low background, and applicability to various samples such as human and mouse.
| Experimental Reagent Information | |
|---|---|
| Reagent Name | Model/Catalog No. |
| Actin (pan) Rabbit Monoclonal Antibody | WR4062 |
| 0.22μm Nitrocellulose Membrane | W3N21 |
| Pre-staining Standard Protein Marker (10-180kDa) | W2100 |
| Dylight 800-conjugated Goat anti-Rabbit Antibody | Cat: WS0446 |
| Sample Lane Corresponding Information | ||
|---|---|---|
| Lane No. | Cell Name | Cell Type (Species Source) |
| Lane1 | HL60 | Human promyelocytic leukemia cell |
| Lane2 | HUVEC | Human umbilical vein endothelial cell |
| Lane3 | Human CAF | Human cancer-associated fibroblast |
| Lane4 | HCT116 | Human colorectal carcinoma |
| Lane5 | A549 | Human lung carcinoma |
| Lane6 | Jurkat | Human T lymphocyte leukemia |
| Lane7 | HT22 | Mouse hippocampal neuronal |
| Lane8 | Thp-1 | Human monocytic leukemia |
| Lane9 | HK-2 | Human proximal tubular epithelial |
| Lane10 | PC-12 | Rat adrenal pheochromocytoma |
| Lane11 | 3T3-L1 | Mouse embryonic fibroblast cells |
| Lane12 | PC-3 | Human prostate adenocarcinoma |
| Lane13 | U-14 | Mouse cervical carcinoma |
| Lane14 | 293F | HEK293 derivative, adapted for suspension culture |
| Key Experimental Conditions | |
|---|---|
| Experimental Step | Specific Parameters |
| Protein Separation (SDS-PAGE) | 4-20% gradient gel, used to separate various whole cell lysates |
| Primary Antibody Incubation | Incubated at 4°C overnight with a primary antibody dilution ratio of 1:500 |
| Secondary Antibody Detection | Dylight 800-conjugated Goat anti-Rabbit Antibody (Cat: WS0446) was used for signal detection |
| Target Protein Band Size Verification | |
|---|---|
| Predicted band size | 42kDa |
| Observed band size | 42kDa |
The experimental data presented earlier fully validates that this Actin (pan) Rabbit Monoclonal Antibody (Catalog Nos. WR4062) stands out as a reliable internal control tool for protein expression research—thanks to its batch consistency enabled by recombinant monoclonal technology, broad species reactivity across human, mouse, and rat samples, and compatibility with multiple applications including Western Blot (WB), Immunohistochemistry (IHC), and Immunofluorescence (IF). Notably, the single 42kDa band observed across 14 distinct cell lines further confirms its ability to address key research pain points such as non-specific band interference and sample compatibility challenges.
If you need to access the antibody’s detailed specifications, real-time stock status, or bulk purchasing options, or if you wish to download the complete experimental protocol (including optimized IHC antigen retrieval steps and IP lysis buffer selection guidelines), please visit our product page directly:Actin (pan) Rabbit Monoclonal Antibody . Our technical support team is also available here to help resolve experimental challenges, supporting your efficient advancement in studies related to actin cytoskeleton regulation, disease mechanisms, and more.
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